Free fatty acids enhance the oxidative damage induced by ethanol metabolism in an in vitro model

Ileana Hernández, Mayra Domínguez-Pérez, Leticia Bucio, Verónica Souza, Roxana U. Miranda, Dahn L. Clemens, Luis Enrique Gomez-Quiroz, María Concepción Gutiérrez-Ruiz

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

In recent years, there has been a growing interest to explore the responsiveness to injury in steatotic hepatocyte. VL-17A cells, which express ADH and Cyp2E1 overloaded with free fatty acids (1 mM of oleic and palmitic acid 2:1) showed an increased oxidative damaged after 24 h free fatty acids treatment when exposed to ethanol (100 mM) for 48 h as a second injury. An increment in reactive oxygen species, determined by DCFH-DA, protein oxidation, and apoptosis were observed although an increase in main antioxidant proteins such as superoxide dismutase 1 and glutathione peroxidase were observed, but failed in gamma-glutamylcysteine synthetase, suggesting a decreased capacity of synthesis of glutathione compared with cells treated only with free fatty acids or ethanol. The increased oxidative stress and toxicity in lipid overloaded VL-17A cells subjected to ethanol exposure were accompanied by increases in Cyp2E1 protein expression. Our data show that lipid loaded in an in vitro model, VL-17A cells, is more susceptible to cell damage and oxidative stress when treated with ethanol.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalFood and Chemical Toxicology
Volume76
DOIs
StatePublished - Feb 1 2015

Keywords

  • Ethanol
  • Hepatocytes
  • Oxidative stress
  • Steatosis
  • VL-17A

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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    Hernández, I., Domínguez-Pérez, M., Bucio, L., Souza, V., Miranda, R. U., Clemens, D. L., Gomez-Quiroz, L. E., & Gutiérrez-Ruiz, M. C. (2015). Free fatty acids enhance the oxidative damage induced by ethanol metabolism in an in vitro model. Food and Chemical Toxicology, 76, 109-115. https://doi.org/10.1016/j.fct.2014.12.005