TY - JOUR
T1 - Free fatty acids enhance the oxidative damage induced by ethanol metabolism in an in vitro model
AU - Hernández, Ileana
AU - Domínguez-Pérez, Mayra
AU - Bucio, Leticia
AU - Souza, Verónica
AU - Miranda, Roxana U.
AU - Clemens, Dahn L.
AU - Gomez-Quiroz, Luis Enrique
AU - Gutiérrez-Ruiz, María Concepción
N1 - Funding Information:
This work was partially funded by a grant from the Consejo Nacional de Ciencia y Tecnología ( CONACYT166042 ); SEP-PROMEP 913026-14612111 , Universidad Autónoma Metropolitana . We thank CONACYT for financial support to Ileana Hernández Reséndiz on her Master in Science studies and Dr. Rocio Ortíz, Depto. Ciencias de la Salud, for the assistance in Flow Cytometry analysis.
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - In recent years, there has been a growing interest to explore the responsiveness to injury in steatotic hepatocyte. VL-17A cells, which express ADH and Cyp2E1 overloaded with free fatty acids (1 mM of oleic and palmitic acid 2:1) showed an increased oxidative damaged after 24 h free fatty acids treatment when exposed to ethanol (100 mM) for 48 h as a second injury. An increment in reactive oxygen species, determined by DCFH-DA, protein oxidation, and apoptosis were observed although an increase in main antioxidant proteins such as superoxide dismutase 1 and glutathione peroxidase were observed, but failed in gamma-glutamylcysteine synthetase, suggesting a decreased capacity of synthesis of glutathione compared with cells treated only with free fatty acids or ethanol. The increased oxidative stress and toxicity in lipid overloaded VL-17A cells subjected to ethanol exposure were accompanied by increases in Cyp2E1 protein expression. Our data show that lipid loaded in an in vitro model, VL-17A cells, is more susceptible to cell damage and oxidative stress when treated with ethanol.
AB - In recent years, there has been a growing interest to explore the responsiveness to injury in steatotic hepatocyte. VL-17A cells, which express ADH and Cyp2E1 overloaded with free fatty acids (1 mM of oleic and palmitic acid 2:1) showed an increased oxidative damaged after 24 h free fatty acids treatment when exposed to ethanol (100 mM) for 48 h as a second injury. An increment in reactive oxygen species, determined by DCFH-DA, protein oxidation, and apoptosis were observed although an increase in main antioxidant proteins such as superoxide dismutase 1 and glutathione peroxidase were observed, but failed in gamma-glutamylcysteine synthetase, suggesting a decreased capacity of synthesis of glutathione compared with cells treated only with free fatty acids or ethanol. The increased oxidative stress and toxicity in lipid overloaded VL-17A cells subjected to ethanol exposure were accompanied by increases in Cyp2E1 protein expression. Our data show that lipid loaded in an in vitro model, VL-17A cells, is more susceptible to cell damage and oxidative stress when treated with ethanol.
KW - Ethanol
KW - Hepatocytes
KW - Oxidative stress
KW - Steatosis
KW - VL-17A
UR - http://www.scopus.com/inward/record.url?scp=84920749415&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920749415&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2014.12.005
DO - 10.1016/j.fct.2014.12.005
M3 - Article
C2 - 25528116
AN - SCOPUS:84920749415
SN - 0278-6915
VL - 76
SP - 109
EP - 115
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -