TY - JOUR
T1 - Free fatty acids enhance the oxidative damage induced by ethanol metabolism in an in vitro model
AU - Hernández, Ileana
AU - Domínguez-Pérez, Mayra
AU - Bucio, Leticia
AU - Souza, Verónica
AU - Miranda, Roxana U.
AU - Clemens, Dahn L.
AU - Gomez-Quiroz, Luis Enrique
AU - Gutiérrez-Ruiz, María Concepción
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - In recent years, there has been a growing interest to explore the responsiveness to injury in steatotic hepatocyte. VL-17A cells, which express ADH and Cyp2E1 overloaded with free fatty acids (1 mM of oleic and palmitic acid 2:1) showed an increased oxidative damaged after 24 h free fatty acids treatment when exposed to ethanol (100 mM) for 48 h as a second injury. An increment in reactive oxygen species, determined by DCFH-DA, protein oxidation, and apoptosis were observed although an increase in main antioxidant proteins such as superoxide dismutase 1 and glutathione peroxidase were observed, but failed in gamma-glutamylcysteine synthetase, suggesting a decreased capacity of synthesis of glutathione compared with cells treated only with free fatty acids or ethanol. The increased oxidative stress and toxicity in lipid overloaded VL-17A cells subjected to ethanol exposure were accompanied by increases in Cyp2E1 protein expression. Our data show that lipid loaded in an in vitro model, VL-17A cells, is more susceptible to cell damage and oxidative stress when treated with ethanol.
AB - In recent years, there has been a growing interest to explore the responsiveness to injury in steatotic hepatocyte. VL-17A cells, which express ADH and Cyp2E1 overloaded with free fatty acids (1 mM of oleic and palmitic acid 2:1) showed an increased oxidative damaged after 24 h free fatty acids treatment when exposed to ethanol (100 mM) for 48 h as a second injury. An increment in reactive oxygen species, determined by DCFH-DA, protein oxidation, and apoptosis were observed although an increase in main antioxidant proteins such as superoxide dismutase 1 and glutathione peroxidase were observed, but failed in gamma-glutamylcysteine synthetase, suggesting a decreased capacity of synthesis of glutathione compared with cells treated only with free fatty acids or ethanol. The increased oxidative stress and toxicity in lipid overloaded VL-17A cells subjected to ethanol exposure were accompanied by increases in Cyp2E1 protein expression. Our data show that lipid loaded in an in vitro model, VL-17A cells, is more susceptible to cell damage and oxidative stress when treated with ethanol.
KW - Ethanol
KW - Hepatocytes
KW - Oxidative stress
KW - Steatosis
KW - VL-17A
UR - http://www.scopus.com/inward/record.url?scp=84920749415&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920749415&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2014.12.005
DO - 10.1016/j.fct.2014.12.005
M3 - Article
C2 - 25528116
AN - SCOPUS:84920749415
SN - 0278-6915
VL - 76
SP - 109
EP - 115
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -