TY - JOUR
T1 - Frequent loss of 9p21 (p16(INK4A)) and other genomic imbalances in human malignant fibrous histiocytoma
AU - Simons, Annet
AU - Schepens, Marga
AU - Jeuken, Judith
AU - Sprenger, Sandra
AU - Van De Zande, Guillaume
AU - Bjerkehagen, Bodil
AU - Forus, Anne
AU - Weibolt, Vines
AU - Molenaar, Ineke
AU - Van Den Berg, Eva
AU - Myklebost, Ola
AU - Bridge, Julia
AU - Van Kessel, Ad Geurts
AU - Suijkerbuijk, Ron
N1 - Funding Information:
The authors thank Angelique Siepman, Daniel Olde Weghuis, Marilu Nelson, Michelle Hess, and Joanne Degenhardt for expert technical assistance. This research was supported by The Dutch Cancer Society (Koningin Wilhelmina Fonds); The Technology Foundation STW (Ministery of Economic Affairs), The Netherlands; The John A. Wiebe Children's Health Care Fund; and the Nebraska State Department of Health LB595.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - To search for new recurrent genetic aberrations in malignant fibrous histiocytoma (MFH), a combination of conventional cytogenetic, comparative genomic hybridization (CGH), and Southern blot analyses was applied to a series of 34 tumors. Cytogenetic analysis revealed the presence of multiple structural and numerical aberrations, including marker chromosomes, telomeric associations, double minutes, and ring chromosomes. The most frequent genomic imbalances in this series of neoplasms as detected by CGH were gains of 1q21-q22 (69%), 17q23-qter (41%), and 20q (66%), and losses of 9p21-pter (55%), 10q (48%), 11q23-qter (55%), and 13q10-q31 (55%). Southern blot analyses with p16(INK4A) (CDKN2A; 9p21) and RB1 (13q14) probes provided clear indications for frequent deletions of these tumor suppressor genes, and as such, substantiated the CGH results. Additionally, examination of the TP53 and MDM2 genes showed frequent loss and amplification, respectively. These data indicate that genes involved in the RB1- and TP53-associated cell cycle regulatory pathways may play prominent roles in the development of human MFH. Copyright (C) 2000 Elsevier Science Inc.
AB - To search for new recurrent genetic aberrations in malignant fibrous histiocytoma (MFH), a combination of conventional cytogenetic, comparative genomic hybridization (CGH), and Southern blot analyses was applied to a series of 34 tumors. Cytogenetic analysis revealed the presence of multiple structural and numerical aberrations, including marker chromosomes, telomeric associations, double minutes, and ring chromosomes. The most frequent genomic imbalances in this series of neoplasms as detected by CGH were gains of 1q21-q22 (69%), 17q23-qter (41%), and 20q (66%), and losses of 9p21-pter (55%), 10q (48%), 11q23-qter (55%), and 13q10-q31 (55%). Southern blot analyses with p16(INK4A) (CDKN2A; 9p21) and RB1 (13q14) probes provided clear indications for frequent deletions of these tumor suppressor genes, and as such, substantiated the CGH results. Additionally, examination of the TP53 and MDM2 genes showed frequent loss and amplification, respectively. These data indicate that genes involved in the RB1- and TP53-associated cell cycle regulatory pathways may play prominent roles in the development of human MFH. Copyright (C) 2000 Elsevier Science Inc.
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U2 - 10.1016/S0165-4608(99)00178-8
DO - 10.1016/S0165-4608(99)00178-8
M3 - Article
C2 - 10748288
AN - SCOPUS:0034656251
SN - 0165-4608
VL - 118
SP - 89
EP - 98
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 2
ER -