Functional loss of chemotactic factor inactivator in the adult respiratory distress syndrome

R. Robbins, R. Maunder, G. Gossman, T. Kendall, L. Hudson, S. Rennard

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The adult respiratory distress syndrome (ARDS) is often characterized by a neutrophilic alveolitis, which may be mediated in part by the neutrophil chemoattractant, C5a. Chemotactic factor inactivator (CFI) can decrease C5a-directed neutrophil chemotaxis. Thus, a loss of CFI activity in the ARDS lung could lead to an increased ability of C5a to attract neutrophils. Lung CFI levels were measured antigenically and functionally in bronchoalveolar lavage (BAL) fluid obtained from 29 patients with ARDS and in 14 normal control subjets. Antigenic levels of CFI were found to be markedly elevated in ARDS BAL fluid (1,855 ± 437 ng/ml) compared with that in normal BAL (29 ± 10 ng/ml, p<0.005), but, in contrast, CFI functional activity was markedly decreased in ARDS BAL fluid compared with that in normal BAL fluid (31 ± 7% inhibition versus 76 ± 5% inhibition, p<0.01). Furthermore, although purified CFI readily inhibited the ability of C5a to attract neutrophils (92% inhibition), this activity was decreased when BAL fluid from patients with ARDS was incubated with CFI (47 ± 10%, p<0.01). These findings suggest that patients with ARDS are functionally deficient in CFI, leading to an increased ability of C5a to attract neutrophils.

Original languageEnglish (US)
Pages (from-to)1463-1468
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume141
Issue number6
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Functional loss of chemotactic factor inactivator in the adult respiratory distress syndrome'. Together they form a unique fingerprint.

  • Cite this