TY - JOUR
T1 - Functional loss of chemotactic factor inactivator in the adult respiratory distress syndrome
AU - Robbins, R.
AU - Maunder, R.
AU - Gossman, G.
AU - Kendall, T.
AU - Hudson, L.
AU - Rennard, S.
PY - 1990
Y1 - 1990
N2 - The adult respiratory distress syndrome (ARDS) is often characterized by a neutrophilic alveolitis, which may be mediated in part by the neutrophil chemoattractant, C5a. Chemotactic factor inactivator (CFI) can decrease C5a-directed neutrophil chemotaxis. Thus, a loss of CFI activity in the ARDS lung could lead to an increased ability of C5a to attract neutrophils. Lung CFI levels were measured antigenically and functionally in bronchoalveolar lavage (BAL) fluid obtained from 29 patients with ARDS and in 14 normal control subjets. Antigenic levels of CFI were found to be markedly elevated in ARDS BAL fluid (1,855 ± 437 ng/ml) compared with that in normal BAL (29 ± 10 ng/ml, p<0.005), but, in contrast, CFI functional activity was markedly decreased in ARDS BAL fluid compared with that in normal BAL fluid (31 ± 7% inhibition versus 76 ± 5% inhibition, p<0.01). Furthermore, although purified CFI readily inhibited the ability of C5a to attract neutrophils (92% inhibition), this activity was decreased when BAL fluid from patients with ARDS was incubated with CFI (47 ± 10%, p<0.01). These findings suggest that patients with ARDS are functionally deficient in CFI, leading to an increased ability of C5a to attract neutrophils.
AB - The adult respiratory distress syndrome (ARDS) is often characterized by a neutrophilic alveolitis, which may be mediated in part by the neutrophil chemoattractant, C5a. Chemotactic factor inactivator (CFI) can decrease C5a-directed neutrophil chemotaxis. Thus, a loss of CFI activity in the ARDS lung could lead to an increased ability of C5a to attract neutrophils. Lung CFI levels were measured antigenically and functionally in bronchoalveolar lavage (BAL) fluid obtained from 29 patients with ARDS and in 14 normal control subjets. Antigenic levels of CFI were found to be markedly elevated in ARDS BAL fluid (1,855 ± 437 ng/ml) compared with that in normal BAL (29 ± 10 ng/ml, p<0.005), but, in contrast, CFI functional activity was markedly decreased in ARDS BAL fluid compared with that in normal BAL fluid (31 ± 7% inhibition versus 76 ± 5% inhibition, p<0.01). Furthermore, although purified CFI readily inhibited the ability of C5a to attract neutrophils (92% inhibition), this activity was decreased when BAL fluid from patients with ARDS was incubated with CFI (47 ± 10%, p<0.01). These findings suggest that patients with ARDS are functionally deficient in CFI, leading to an increased ability of C5a to attract neutrophils.
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U2 - 10.1164/ajrccm/141.6.1463
DO - 10.1164/ajrccm/141.6.1463
M3 - Article
C2 - 2350087
AN - SCOPUS:0025367831
VL - 141
SP - 1463
EP - 1468
JO - American Review of Respiratory Disease
JF - American Review of Respiratory Disease
SN - 1073-449X
IS - 6
ER -