Fusarium graminearum from Expression Analysis to Functional Assays

Heather E. Hallen-Adams, Brad L. Cavinder, Frances Trail

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Scopus citations

Abstract

Fusarium graminearum, the causal agent of head blight of wheat, was the third filamentous fungus to have a completed genome sequence. Since the release of the genome sequence in 2003, F. graminearum has become a model for studies of genomics and transcriptomics, mycotoxins, fungal population genetics, gene function, and sexual development. Herein we present the techniques we have used in our laboratory to perform expression analyses on life cycle stages of F. graminearum and techniques to functionally characterize those genes identified as potentially interesting.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages79-101
Number of pages23
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume722
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Affymetrix
  • Gibberella zeae
  • Microarrays
  • Protoplasts
  • RNA extraction
  • Transcriptomics
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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