GCSF-R expression in myelodysplastic and myeloproliferative disorders and blast dysmaturation in CML

Vidya Sagar Hanumanthu, Samuel J. Pirruccello

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objectives: To characterize granulocyte colony-stimulating factor receptor (CD114) expression in normal (n = 20), myelodysplastic (n = 34), and chronic myelogenous leukemia (CML; n = 5) bone marrow by flow cytometry. Methods: Clinical bone marrow samples were analyzed using CD33/CD114/CD34/CD117/CD45. CD114 density (mean fluorescence intensity) and cellular distribution were evaluated on early blasts (CD33-), late blasts (CD33+), promyelocytes, and granulocytes. Results: Normal CD114 acquisition occurred on early blasts, peaked on promyelocytes, and decreased on granulocytes. Forty percent of CD34+ blasts expressed CD114 and one-third were early blasts. In myelodysplastic syndromes, altered CD114 distribution was more informative than density changes. In CML, CD114 density was significantly decreased on early blasts and expression was essentially limited to late blasts. We observed a specific blast dysmaturation pattern in CML involving CD33, CD34, and CD114 that was 83% sensitive and 100% specific in initial diagnosis. Conclusions: CD114 provides useful additional detail in phenotypic assessment of hematopoietic precursor maturation.

Original languageEnglish (US)
Pages (from-to)155-164
Number of pages10
JournalAmerican journal of clinical pathology
Volume140
Issue number2
DOIs
StatePublished - Aug 1 2013

Keywords

  • CD114
  • Chronic myelogenous leukemia
  • Flow cytometry
  • GCSF-R
  • Myelodysplastic syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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