TY - JOUR
T1 - GDNF regulates the Aβ-induced endoplasmic reticulum stress response in rabbit hippocampus by inhibiting the activation of gadd 153 and the JNK and ERK kinases
AU - Ghribi, Othman
AU - Herman, Mary M.
AU - Pramoonjago, Patcharin
AU - Spaulding, Natalie K.
AU - Savory, John
N1 - Funding Information:
Supported by the Virginia Center on Aging. We thank Dr. Peter Davies for his gift of antibody to PHF1.
PY - 2004/7
Y1 - 2004/7
N2 - Glial cell line-derived neurotrophic factor (GDNF) is a potent survival agent for neurons, however, its effect on Aβ-evoked neuronal death has not been examined. We show that the injection of Aβ into New Zealand white rabbit brain activates the endoplasmic reticulum (ER) chaperones, grp 78 and grp 94, and the transcription factor, gadd 153. These effects correlate with the activation of JNK and ERK as well as of microglia and with the phosphorylation of tau protein. Treatment with GDNF inhibits the activation of gadd 153, reduces the phosphorylation of JNK, abolishes the phosphorylation of ERK, prevents microglial activation, greatly reduces apoptotic cells, and does not affect the phosphorylation of tau. Our data suggest that the tau hyperphosphorylation and apoptosis triggered by Aβ are two independent events, and that the neuroprotective effect of GDNF against Aβ may result either directly by the inhibition of ER stress or indirectly through the inhibition of JNK and ERK activation.
AB - Glial cell line-derived neurotrophic factor (GDNF) is a potent survival agent for neurons, however, its effect on Aβ-evoked neuronal death has not been examined. We show that the injection of Aβ into New Zealand white rabbit brain activates the endoplasmic reticulum (ER) chaperones, grp 78 and grp 94, and the transcription factor, gadd 153. These effects correlate with the activation of JNK and ERK as well as of microglia and with the phosphorylation of tau protein. Treatment with GDNF inhibits the activation of gadd 153, reduces the phosphorylation of JNK, abolishes the phosphorylation of ERK, prevents microglial activation, greatly reduces apoptotic cells, and does not affect the phosphorylation of tau. Our data suggest that the tau hyperphosphorylation and apoptosis triggered by Aβ are two independent events, and that the neuroprotective effect of GDNF against Aβ may result either directly by the inhibition of ER stress or indirectly through the inhibition of JNK and ERK activation.
KW - Aβ
KW - ERK
KW - Endoplasmic reticulum
KW - GDNF
KW - JNK
KW - Tau
KW - gadd 153
KW - grp 78
KW - grp 94
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U2 - 10.1016/j.nbd.2004.04.002
DO - 10.1016/j.nbd.2004.04.002
M3 - Article
C2 - 15193298
AN - SCOPUS:2942586899
SN - 0969-9961
VL - 16
SP - 417
EP - 427
JO - Neurobiology of Disease
JF - Neurobiology of Disease
IS - 2
ER -