GDNF regulates the Aβ-induced endoplasmic reticulum stress response in rabbit hippocampus by inhibiting the activation of gadd 153 and the JNK and ERK kinases

Othman Ghribi, Mary M. Herman, Patcharin Pramoonjago, Natalie K. Spaulding, John Savory

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival agent for neurons, however, its effect on Aβ-evoked neuronal death has not been examined. We show that the injection of Aβ into New Zealand white rabbit brain activates the endoplasmic reticulum (ER) chaperones, grp 78 and grp 94, and the transcription factor, gadd 153. These effects correlate with the activation of JNK and ERK as well as of microglia and with the phosphorylation of tau protein. Treatment with GDNF inhibits the activation of gadd 153, reduces the phosphorylation of JNK, abolishes the phosphorylation of ERK, prevents microglial activation, greatly reduces apoptotic cells, and does not affect the phosphorylation of tau. Our data suggest that the tau hyperphosphorylation and apoptosis triggered by Aβ are two independent events, and that the neuroprotective effect of GDNF against Aβ may result either directly by the inhibition of ER stress or indirectly through the inhibition of JNK and ERK activation.

Original languageEnglish (US)
Pages (from-to)417-427
Number of pages11
JournalNeurobiology of Disease
Volume16
Issue number2
DOIs
StatePublished - Jul 2004

Keywords

  • ERK
  • Endoplasmic reticulum
  • GDNF
  • JNK
  • Tau
  • gadd 153
  • grp 78
  • grp 94

ASJC Scopus subject areas

  • Neurology

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