Gene expression profiles in acute myeloid leukemia with common translocations using SAGE

Sanggyu Lee, Jianjun Chen, Guolin Zhou, Run Zhang Shi, Gerard G. Bouffard, Masha Kocherginsky, Xijin Ge, Miao Sun, Nimanthi Jayathilaka, Yeong Cheol Kim, Neelmini Emmanuel, Stefan K. Bohlander, Mark Minden, Justin Kline, Ozden Ozer, Richard A. Larson, Michelle M. LeBeau, Eric D. Green, Jeffery Trent, Theodore KarrisonPiu Paul Liu, San Ming Wang, Janet D. Rowley

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Identification of the specific cytogenetic abnormality is one of the critical steps for classification of acute myeloblastic leukemia (AML) which influences the selection of appropriate therapy and provides information about disease prognosis. However at present, the genetic complexity of AML is only partially understood. To obtain a comprehensive, unbiased, quantitative measure, we performed serial analysis of gene expression (SAGE) on CD15+ myeloid progenitor cells from 22 AML patients who had four of the most common translocations, namely t(8;21), t(15;17), t(9;11), and inv(16). The quantitative data provide clear evidence that the major change in all these translocation-carrying leukemias is a decrease in expression of the majority of transcripts compared with normal CD15+ cells. From a total of 1,247,535 SAGE tags, we identified 2,604 transcripts whose expression was significantly altered in these leukemias compared with normal myeloid progenitor cells. The gene ontology of the 1,110 transcripts that matched known genes revealed that each translocation had a uniquely altered profile in various functional categories including regulation of transcription, cell cycle, protein synthesis, and apoptosis. Our global analysis of gene expression of common translocations in AML can focus attention on the function of the genes with altered expression for future biological studies as well as highlight genes/pathways for more specifically targeted therapy.

Original languageEnglish (US)
Pages (from-to)1030-1035
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number4
StatePublished - Jan 24 2006
Externally publishedYes


  • Diagnostic microarray
  • Hematopoietic cell differention

ASJC Scopus subject areas

  • General


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