Gene family encoding the major toxins of lethal Amanita mushrooms

Heather E. Hallen, Hong Luo, John S. Scott-Craig, Jonathan D. Walton

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

Amatoxins, the lethal constituents of poisonous mushrooms in the genus Amanita, are bicyclic octapeptides. Two genes in A. bisporigera, AMA1 and PHA1, directly encode α-amanitin, an amatoxin, and the related bicyclic heptapeptide phallacidin, a phallotoxin, indicating that these compounds are synthesized on ribosomes and not by nonribosomal peptide synthetases. α-Amanitin and phallacidin are synthesized as proproteins of 35 and 34 amino acids, respectively, from which they are predicted to be cleaved by a prolyl oligopeptidase. AMA1 and PHA1 are present in other toxic species of Amanita section Phalloidae but are absent from nontoxic species in other sections. The genomes of A. bisporigera and A. phalloides contain multiple sequences related to AMA1 and PHA1. The predicted protein products of this family of genes are characterized by a hypervariable "toxin" region capable of encoding a wide variety of peptides of 7-10 amino acids flanked by conserved sequences. Our results suggest that these fungi have a broad capacity to synthesize cyclic peptides on ribosomes.

Original languageEnglish (US)
Pages (from-to)19097-19101
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number48
DOIs
StatePublished - Nov 27 2007

Keywords

  • Amanitin
  • Amatoxin
  • Cyclic peptide
  • Phalloidin
  • Phallotoxin

ASJC Scopus subject areas

  • General

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