Gene replacement and retrieval with recombinant M13mp bacteriophages

P. Blum, D. Holzschu, H. S. Kwan, D. Riggs, S. Artz

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

We have developed an allele exchange system for shuttling sequences of DNA to and from their original chromosomal loci. Cloned segments of the histidine operon of Salmonella typhimurium and the lactose operon of Escherichia coli served as target sequences and were used to develop the system. Replacement and retrieval of target sequences used the phase M13mp vectors and proceeded through an M13 lysogen intermediate. The intermediates and products of allele exchange were characterized by genetic and hybridization analyses. Several unique properties of M13 lysogens were exploited to devise positive selections to detect integration and excision. These positive selections were used to manipulate phenotypically silent alleles.

Original languageEnglish (US)
Pages (from-to)538-546
Number of pages9
JournalJournal of bacteriology
Volume171
Issue number1
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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