Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis

Jessica N. Snowden

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Animal models are valuable tools for investigating the in vivo pathogenesis of Staphylococcus epidermidis infections. Here, we present the procedure for generating a central nervous system catheter-associated infection in a mouse, to model the central nervous system shunt infections that frequently complicate the treatment of hydrocephalus in humans. This model uses stereotactic guidance to place silicone catheters, pre-coated with S. epidermidis, into the lateral ventricles of mice. This results in a catheter-associated infection in the brain, with concomitant illness and inflammation. This animal model is a valuable tool for evaluating the pathogenesis of bacterial infection in the central nervous system, the immune response to these infections and potential treatment options.

Original languageEnglish (US)
Title of host publicationStaphylococcus Epidermidis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages193-198
Number of pages6
ISBN (Print)9781627037358
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1106
ISSN (Print)1064-3745

Keywords

  • Catheter infection
  • Central nervous system
  • Mouse model
  • Shunt infection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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