Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking

Ruthellen H. Anderson; Jason G. Kerkvliet; Jaelin J. Otta; Alan D. Ross; Patricia C. Leiferman; Adam D. Hoppe; Kevin R. Francis

doi:10.1016/j.scr.2018.10.001

Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking

Ruthellen H. Anderson, Jason G. Kerkvliet, Jaelin J. Otta, Alan D. Ross, Patricia C. Leiferman, Adam D. Hoppe, Kevin R. Francis

Great Plains IDeA-CTR

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.

Original language	English (US)
Pages (from-to)	95-99
Number of pages	5
Journal	Stem Cell Research
Volume	33
DOIs	https://doi.org/10.1016/j.scr.2018.10.001
State	Published - Dec 2018

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1016/j.scr.2018.10.001

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title = "Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking",

abstract = "The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.",

author = "Anderson, {Ruthellen H.} and Kerkvliet, {Jason G.} and Otta, {Jaelin J.} and Ross, {Alan D.} and Leiferman, {Patricia C.} and Hoppe, {Adam D.} and Francis, {Kevin R.}",

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year = "2018",

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doi = "10.1016/j.scr.2018.10.001",

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T1 - Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking

AU - Anderson, Ruthellen H.

AU - Kerkvliet, Jason G.

AU - Otta, Jaelin J.

AU - Ross, Alan D.

AU - Leiferman, Patricia C.

AU - Hoppe, Adam D.

AU - Francis, Kevin R.

PY - 2018/12

Y1 - 2018/12

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AB - The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.

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Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking

Abstract

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