TY - JOUR
T1 - Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking
AU - Anderson, Ruthellen H.
AU - Kerkvliet, Jason G.
AU - Otta, Jaelin J.
AU - Ross, Alan D.
AU - Leiferman, Patricia C.
AU - Hoppe, Adam D.
AU - Francis, Kevin R.
N1 - Funding Information:
This research was supported by the National Institutes of Health (grant numbers P20GM103620 and P20GM103548 ) and the National Science Foundation /EPSCoR Cooperative Agreement #IIA-1355423 , the South Dakota Research and Innovation Center , BioSNTR , and by the State of South Dakota BOR CRGP .
Funding Information:
This research was supported by the National Institutes of Health (grant numbers P20GM103620 and P20GM103548) and the National Science Foundation/EPSCoR Cooperative Agreement #IIA-1355423, the South Dakota Research and Innovation Center, BioSNTR, and by the State of South Dakota BOR CRGP.
Publisher Copyright:
© 2018 The Authors
PY - 2018/12
Y1 - 2018/12
N2 - The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.
AB - The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.
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U2 - 10.1016/j.scr.2018.10.001
DO - 10.1016/j.scr.2018.10.001
M3 - Article
C2 - 30340091
AN - SCOPUS:85054881910
SN - 1873-5061
VL - 33
SP - 95
EP - 99
JO - Stem Cell Research
JF - Stem Cell Research
ER -