Generation of an infectious cDNA of a highly cardiovirulent coxsackievirus B3(CVB3(m)) and comparison to other infectious CVB3 CDNAs

C. Lee, E. Maull, N. Chapman, S. Tracy, J. Wood, C. Gauntt

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

An infectious cDNA of a highly myocarditic coxsackievirus B3 (CVB3(m); Nancy strain) was cloned. Sequence data revealed 43 extra non-viral nucleotides upstream of the initial 5' sequence. However, the authentic 5' end sequence was maintained during replication of viral RNA transfected into HeLa cells, suggesting the RNA synthesizing complex edits the picornaviral 5' terminus sequence. Nucleotide sequences of the 5' nontranslated region and the capsid protein gene sequence of CVB3(m) were compared with the published sequences of five other CVB3 Nancy strains and two main lineages were found. In comparative assays for cardiovirulence, three of four CVB3 tested were cardiovirulent in adolescent male CD-1 mice. Only one of the three available CVB3 strains was neutralized with several anti-CVB3(m) monoclonal antibodies, suggesting that mutations in the surface epitopes of the capsid polypeptides contribute to antigenic drift within the serotype, perhaps in part through immunoselective pressures. Thus, phenotypic diversity of CVB3 within the prototype Nancy strain is an example of RNA viruses adapting to changing environments (cells, mice and humans) through mutations and selective pressure.

Original languageEnglish (US)
Pages (from-to)225-235
Number of pages11
JournalVirus Research
Volume50
Issue number2
DOIs
StatePublished - Aug 1997

Keywords

  • Cardiovirulence
  • Coxsackievirus B3
  • Molecular evolution
  • RNA editing
  • Selective pressure
  • cDNA cloning

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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