Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D

K. W. Bayles, J. J. Iandolo

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

The gene (entD) encoding staphylocccal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this fragment revealed an open reading frame that encoded a 258-amino-acid protein that possessed a 30-amino-acid signal peptide. The 228-amino-acid mature polypeptide had a molecular weight of 26,360 and contained a high degree of sequence similarity to the other staphylococcal enterotoxins. S1 nuclease mapping showed that transcription of entD was initiated 266 nucleotides upstream from the translation start codon. The entD gene was also shown to be activated by the staphylococcal regulatory element known as agr.

Original languageEnglish (US)
Pages (from-to)4799-4806
Number of pages8
JournalJournal of bacteriology
Volume171
Issue number9
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D'. Together they form a unique fingerprint.

  • Cite this