Genetic Code Expansion Through Quadruplet Codon Decoding

Jiantao Guo, Wei Niu

Research output: Contribution to journalReview articlepeer-review

7 Scopus citations


Noncanonical amino acid mutagenesis has emerged as a powerful tool for the study of protein structure and function. While triplet nonsense codons, especially the amber codon, have been widely employed, quadruplet codons have attracted attention for the potential of creating additional blank codons for noncanonical amino acids mutagenesis. In this review, we discuss methodologies and applications of quadruplet codon decoding in genetic code expansion both in vitro and in vivo.

Original languageEnglish (US)
Article number167346
JournalJournal of Molecular Biology
Issue number8
StatePublished - Apr 30 2022


  • +1 frameshift
  • four-base codon
  • genetic code
  • non-canonical amino acids
  • quadruplet codon

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology


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