Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

Xinyuan He; Bin Ma; Yan Chen; Jiantao Guo; Wei Niu

doi:10.1039/d2cc01578a

Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

Xinyuan He, Bin Ma, Yan Chen, Jiantao Guo, Wei Niu

Chemistry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, p-carboxymethyl-l-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies by removing complications caused by the dynamic interconversion between the phosphorylated and non-phosphorylated isoforms of a protein.

Original language	English (US)
Journal	Chemical Communications
DOIs	https://doi.org/10.1039/d2cc01578a
State	Accepted/In press - 2022

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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10.1039/d2cc01578a

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title = "Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells",

abstract = "Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, p-carboxymethyl-l-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies by removing complications caused by the dynamic interconversion between the phosphorylated and non-phosphorylated isoforms of a protein.",

author = "Xinyuan He and Bin Ma and Yan Chen and Jiantao Guo and Wei Niu",

note = "Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry",

year = "2022",

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language = "English (US)",

journal = "Chemical Communications",

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T1 - Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

AU - He, Xinyuan

AU - Ma, Bin

AU - Chen, Yan

AU - Guo, Jiantao

AU - Niu, Wei

PY - 2022

Y1 - 2022

N2 - Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, p-carboxymethyl-l-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies by removing complications caused by the dynamic interconversion between the phosphorylated and non-phosphorylated isoforms of a protein.

AB - Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, p-carboxymethyl-l-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies by removing complications caused by the dynamic interconversion between the phosphorylated and non-phosphorylated isoforms of a protein.

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U2 - 10.1039/d2cc01578a

DO - 10.1039/d2cc01578a

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SN - 1359-7345

JO - Chemical Communications

JF - Chemical Communications

ER -

Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

Abstract

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