TY - JOUR
T1 - Genetic evaluation of candidate genes for the Mom1 modifier of intestinal neoplasia in mice
AU - Gould, Karen A.
AU - Luongo, Cindy
AU - Moser, Amy R.
AU - McNeley, Melanie K.
AU - Borenstein, Natalie
AU - Shedlovsky, Alexandra
AU - Dove, William F.
AU - Hong, Karen
AU - Dietrich, William F.
AU - Lander, Eric S.
PY - 1996/12
Y1 - 1996/12
N2 - As genetic mapping of quantitative trait loci (QTL) becomes routine, the challenge is to identify the underlying genes. This paper develops rigorous genetic tests evaluation of candidate genes for a QTL, involving determination of allelic status in inbred strains and fine-structure genetic mapping. For the Mom1 modifier of intestinal adenomas caused by Apc(Min), these tests are used to evaluate two candidate genes: Pla2g2a, a secretory phospholipase, and Rap1GAP, a GTPase activating protein. Rap1GAP passes the first test but is excluded by a single fine-structure recombinant. Pla2g2a passes both tests and is a strong candidate for Mom1. Significantly, we also find that Apc(Min)-induced adenomas remain heterozygous for the Mom1 region, consistent with Mom1 acting outside the tumor lineage and encoding a secreted product.
AB - As genetic mapping of quantitative trait loci (QTL) becomes routine, the challenge is to identify the underlying genes. This paper develops rigorous genetic tests evaluation of candidate genes for a QTL, involving determination of allelic status in inbred strains and fine-structure genetic mapping. For the Mom1 modifier of intestinal adenomas caused by Apc(Min), these tests are used to evaluate two candidate genes: Pla2g2a, a secretory phospholipase, and Rap1GAP, a GTPase activating protein. Rap1GAP passes the first test but is excluded by a single fine-structure recombinant. Pla2g2a passes both tests and is a strong candidate for Mom1. Significantly, we also find that Apc(Min)-induced adenomas remain heterozygous for the Mom1 region, consistent with Mom1 acting outside the tumor lineage and encoding a secreted product.
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M3 - Article
C2 - 8978063
AN - SCOPUS:12644310314
SN - 0016-6731
VL - 144
SP - 1777
EP - 1785
JO - Genetics
JF - Genetics
IS - 4
ER -