Genetic evaluation of candidate genes for the Mom1 modifier of intestinal neoplasia in mice

Karen A. Gould, Cindy Luongo, Amy R. Moser, Melanie K. McNeley, Natalie Borenstein, Alexandra Shedlovsky, William F. Dove, Karen Hong, William F. Dietrich, Eric S. Lander

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

As genetic mapping of quantitative trait loci (QTL) becomes routine, the challenge is to identify the underlying genes. This paper develops rigorous genetic tests evaluation of candidate genes for a QTL, involving determination of allelic status in inbred strains and fine-structure genetic mapping. For the Mom1 modifier of intestinal adenomas caused by Apc(Min), these tests are used to evaluate two candidate genes: Pla2g2a, a secretory phospholipase, and Rap1GAP, a GTPase activating protein. Rap1GAP passes the first test but is excluded by a single fine-structure recombinant. Pla2g2a passes both tests and is a strong candidate for Mom1. Significantly, we also find that Apc(Min)-induced adenomas remain heterozygous for the Mom1 region, consistent with Mom1 acting outside the tumor lineage and encoding a secreted product.

Original languageEnglish (US)
Pages (from-to)1777-1785
Number of pages9
JournalGenetics
Volume144
Issue number4
StatePublished - Dec 1 1996

ASJC Scopus subject areas

  • Genetics

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    Gould, K. A., Luongo, C., Moser, A. R., McNeley, M. K., Borenstein, N., Shedlovsky, A., Dove, W. F., Hong, K., Dietrich, W. F., & Lander, E. S. (1996). Genetic evaluation of candidate genes for the Mom1 modifier of intestinal neoplasia in mice. Genetics, 144(4), 1777-1785.