Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae

Soo Lee Hyun, Jiantao Guo, Edward A. Lemke, Romerson D. Dimla, Peter G. Schultz

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

(Graph Presented) Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.

Original languageEnglish (US)
Pages (from-to)12921-12923
Number of pages3
JournalJournal of the American Chemical Society
Volume131
Issue number36
DOIs
StatePublished - Sep 16 2009

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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