Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae

Soo Lee Hyun; Jiantao Guo; Edward A. Lemke; Romerson D. Dimla; Peter G. Schultz

doi:10.1021/ja904896s

Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae

Soo Lee Hyun, Jiantao Guo, Edward A. Lemke, Romerson D. Dimla, Peter G. Schultz

Chemistry

Research output: Contribution to journal › Article

113 Scopus citations

Abstract

(Graph Presented) Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.

Original language	English (US)
Pages (from-to)	12921-12923
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	131
Issue number	36
DOIs	https://doi.org/10.1021/ja904896s
State	Published - Sep 16 2009

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja904896s

Fingerprint Dive into the research topics of 'Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this

Hyun, S. L., Guo, J., Lemke, E. A., Dimla, R. D., & Schultz, P. G. (2009). Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae. Journal of the American Chemical Society, 131(36), 12921-12923. https://doi.org/10.1021/ja904896s

@article{d9681de8d8854a0cb7dddc6d416dbff5,

title = "Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae",

abstract = "(Graph Presented) Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.",

author = "Hyun, {Soo Lee} and Jiantao Guo and Lemke, {Edward A.} and Dimla, {Romerson D.} and Schultz, {Peter G.}",

year = "2009",

month = sep,

day = "16",

doi = "10.1021/ja904896s",

language = "English (US)",

volume = "131",

pages = "12921--12923",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "36",

}

TY - JOUR

T1 - Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae

AU - Hyun, Soo Lee

AU - Guo, Jiantao

AU - Lemke, Edward A.

AU - Dimla, Romerson D.

AU - Schultz, Peter G.

PY - 2009/9/16

Y1 - 2009/9/16

N2 - (Graph Presented) Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.

AB - (Graph Presented) Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.

UR - http://www.scopus.com/inward/record.url?scp=70349158729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349158729&partnerID=8YFLogxK

U2 - 10.1021/ja904896s

DO - 10.1021/ja904896s

M3 - Article

C2 - 19702307

AN - SCOPUS:70349158729

VL - 131

SP - 12921

EP - 12923

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 36

ER -