Genetic variation in pneumocystis carinii isolates from different geographic regions: Implications for transmission

Charles B. Beard; Jane L. Carter; Scott P. Keely; Laurence Huang; Norman J. Pieniazek; Iaci N.S. Moura; Jacquelin M. Roberts; Allen W. Hightower; Michelle S. Bens; Amanda R. Freeman; Sherline Lee; James R. Stringer; Jeffrey S. Duchin; Carlos Del Rio; David Rimland; Robert P. Baughman; Deborah A. Levy; Vance J. Dietz; Paul Simon; Thomas R. Navin

doi:10.3201/eid0603.000306

Genetic variation in pneumocystis carinii isolates from different geographic regions: Implications for transmission

Charles B. Beard, Jane L. Carter, Scott P. Keely, Laurence Huang, Norman J. Pieniazek, Iaci N.S. Moura, Jacquelin M. Roberts, Allen W. Hightower, Michelle S. Bens, Amanda R. Freeman, Sherline Lee, James R. Stringer, Jeffrey S. Duchin, Carlos Del Rio, David Rimland, Robert P. Baughman, Deborah A. Levy, Vance J. Dietz, Paul Simon, Thomas R. Navin

Research output: Contribution to journal › Article › peer-review

214 Scopus citations

Abstract

To study transmission patterns of Pneumocystis carinii pneumonia (PCP) in persons with AIDS, we evaluated P. carinii isolates from patients in five U.S. cities for variation at two independent genetic loci, the mitochondrial large subunit rRNA and dihydropteroate synthase. Fourteen unique multilocus genotypes were observed in 191 isolates that were examined at both loci. Mixed infections, accounting for 17.8% of cases, were associated with primary PCP. Genotype frequency distribution patterns varied by patients' place of diagnosis but not by place of birth. Genetic variation at the two loci suggests three probable characteristics of transmission: that most cases of PCP do not result from infections acquired early in life, that infections are actively acquired from a relatively common source (humans or the environment), and that humans, while not necessarily involved in direct infection of other humans, are nevertheless important in the transmission cycle of P. carinii f. sp. hominis.

Original language	English (US)
Pages (from-to)	265-272
Number of pages	8
Journal	Emerging infectious diseases
Volume	6
Issue number	3
DOIs	https://doi.org/10.3201/eid0603.000306
State	Published - 2000
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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Beard, C. B., Carter, J. L., Keely, S. P., Huang, L., Pieniazek, N. J., Moura, I. N. S., Roberts, J. M., Hightower, A. W., Bens, M. S., Freeman, A. R., Lee, S., Stringer, J. R., Duchin, J. S., Del Rio, C., Rimland, D., Baughman, R. P., Levy, D. A., Dietz, V. J., Simon, P., & Navin, T. R. (2000). Genetic variation in pneumocystis carinii isolates from different geographic regions: Implications for transmission. Emerging infectious diseases, 6(3), 265-272. https://doi.org/10.3201/eid0603.000306

Beard, CB, Carter, JL, Keely, SP, Huang, L, Pieniazek, NJ, Moura, INS, Roberts, JM, Hightower, AW, Bens, MS, Freeman, AR, Lee, S, Stringer, JR, Duchin, JS, Del Rio, C, Rimland, D, Baughman, RP, Levy, DA, Dietz, VJ, Simon, P & Navin, TR 2000, 'Genetic variation in pneumocystis carinii isolates from different geographic regions: Implications for transmission', Emerging infectious diseases, vol. 6, no. 3, pp. 265-272. https://doi.org/10.3201/eid0603.000306

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title = "Genetic variation in pneumocystis carinii isolates from different geographic regions: Implications for transmission",

abstract = "To study transmission patterns of Pneumocystis carinii pneumonia (PCP) in persons with AIDS, we evaluated P. carinii isolates from patients in five U.S. cities for variation at two independent genetic loci, the mitochondrial large subunit rRNA and dihydropteroate synthase. Fourteen unique multilocus genotypes were observed in 191 isolates that were examined at both loci. Mixed infections, accounting for 17.8% of cases, were associated with primary PCP. Genotype frequency distribution patterns varied by patients' place of diagnosis but not by place of birth. Genetic variation at the two loci suggests three probable characteristics of transmission: that most cases of PCP do not result from infections acquired early in life, that infections are actively acquired from a relatively common source (humans or the environment), and that humans, while not necessarily involved in direct infection of other humans, are nevertheless important in the transmission cycle of P. carinii f. sp. hominis.",

author = "Beard, {Charles B.} and Carter, {Jane L.} and Keely, {Scott P.} and Laurence Huang and Pieniazek, {Norman J.} and Moura, {Iaci N.S.} and Roberts, {Jacquelin M.} and Hightower, {Allen W.} and Bens, {Michelle S.} and Freeman, {Amanda R.} and Sherline Lee and Stringer, {James R.} and Duchin, {Jeffrey S.} and {Del Rio}, Carlos and David Rimland and Baughman, {Robert P.} and Levy, {Deborah A.} and Dietz, {Vance J.} and Paul Simon and Navin, {Thomas R.}",

year = "2000",

doi = "10.3201/eid0603.000306",

language = "English (US)",

volume = "6",

pages = "265--272",

journal = "Emerging infectious diseases",

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T1 - Genetic variation in pneumocystis carinii isolates from different geographic regions

T2 - Implications for transmission

AU - Beard, Charles B.

AU - Carter, Jane L.

AU - Keely, Scott P.

AU - Huang, Laurence

AU - Pieniazek, Norman J.

AU - Moura, Iaci N.S.

AU - Roberts, Jacquelin M.

AU - Hightower, Allen W.

AU - Bens, Michelle S.

AU - Freeman, Amanda R.

AU - Lee, Sherline

AU - Stringer, James R.

AU - Duchin, Jeffrey S.

AU - Del Rio, Carlos

AU - Rimland, David

AU - Baughman, Robert P.

AU - Levy, Deborah A.

AU - Dietz, Vance J.

AU - Simon, Paul

AU - Navin, Thomas R.

PY - 2000

Y1 - 2000

N2 - To study transmission patterns of Pneumocystis carinii pneumonia (PCP) in persons with AIDS, we evaluated P. carinii isolates from patients in five U.S. cities for variation at two independent genetic loci, the mitochondrial large subunit rRNA and dihydropteroate synthase. Fourteen unique multilocus genotypes were observed in 191 isolates that were examined at both loci. Mixed infections, accounting for 17.8% of cases, were associated with primary PCP. Genotype frequency distribution patterns varied by patients' place of diagnosis but not by place of birth. Genetic variation at the two loci suggests three probable characteristics of transmission: that most cases of PCP do not result from infections acquired early in life, that infections are actively acquired from a relatively common source (humans or the environment), and that humans, while not necessarily involved in direct infection of other humans, are nevertheless important in the transmission cycle of P. carinii f. sp. hominis.

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Genetic variation in pneumocystis carinii isolates from different geographic regions: Implications for transmission

Abstract

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