Genome assembly of the Brassicaceae diploid Orychophragmus violaceus reveals complex whole-genome duplication and evolution of dihydroxy fatty acid metabolism

Fan Huang, Peng Chen, Xinyu Tang, Ting Zhong, Taihua Yang, Chinedu Charles Nwafor, Chao Yang, Xianhong Ge, Hong An, Zaiyun Li, Edgar B. Cahoon, Chunyu Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Orychophragmus violaceus is a Brassicaceae species widely cultivated in China, particularly as a winter cover crop in northern China because of its low-temperature tolerance and low water demand. Recently, O. violaceus has also been cultivated as a potential industrial oilseed crop because of its abundant 24-carbon dihydroxy fatty acids (diOH-FAs), which contribute to superior high-temperature lubricant properties. In this study, we performed de novo assembly of the O. violaceus genome. Whole-genome synteny analysis of the genomes of its relatives demonstrated that O. violaceus is a diploid that has undergone an extra whole-genome duplication (WGD) after the Brassicaceae-specific α-WGD event, with a basic chromosome number of x = 12. Formation of diOH-FAs is hypothesized to have occurred after the WGD event. Based on the genome and the transcriptome data from multiple stages of seed development, we predicted that OvDGAT1-1 and OvDGAT1-2 are candidate genes for the regulation of diOH-FA storage in O. violaceus seeds. These results may greatly facilitate the development of heat-tolerant and eco-friendly plant-based lubricants using O. violaceus seed oil and improve our understanding of the genomic evolution of Brassicaceae.

Original languageEnglish (US)
Article number100432
JournalPlant Communications
Volume4
Issue number2
DOIs
StatePublished - Mar 13 2023

Keywords

  • Brassicaceae
  • Orychophragmus violaceus
  • dihydroxy fatty acids
  • genome evolution
  • lubricant oil
  • oilseed
  • polyestolides

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Cell Biology

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