Genome diversity among regional populations of Francisella tularensis subspecies tularensis and Francisella tularensis subspecies holarctica isolated from the US

M. M. Samrakandi; C. Zhang; M. Zhang; J. Nietfeldt; J. Kim; P. C. Iwen; M. E. Olson; P. D. Fey; G. E. Duhamel; S. H. Hinrichs; J. D. Cirillo; A. K. Benson

doi:10.1016/j.femsle.2004.06.008

Genome diversity among regional populations of Francisella tularensis subspecies tularensis and Francisella tularensis subspecies holarctica isolated from the US

M. M. Samrakandi, C. Zhang, M. Zhang, J. Nietfeldt, J. Kim, P. C. Iwen, M. E. Olson, P. D. Fey, G. E. Duhamel, S. H. Hinrichs, J. D. Cirillo, A. K. Benson

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28 Scopus citations

Abstract

Francisella tularensis is a highly infectious facultative intracellular pathogen that is considered a potential agent of bioterrorism. Four different F. tularensis subspecies have been identified and they appear to display different ecological and virulence characteristics as well as differences in geographical distribution. One simple explanation for the variation in ecological and virulence characteristics is that they are conferred by differences in genome content. To characterize genome content among stains isolated from United States, we have used a DNA microarray designed from a shotgun library of a reference strain. Polymorphisms distributed among polyphyletic sets of strains was the most common pattern of genome alteration observed, indicating that strain-specific genome variability is significant. Nonetheless, 13 different contiguous segments of the genome were found to be missing exclusively in each of the subsp. holarctica strains tested. All 13 are associated with repeat sequences or transposases that could promote insertion/deletion events. Comparison of the live vaccine strain to other holarctica strains also identified three regions that are absent exclusively in the live vaccine strain derived from holarctica.

Original language	English (US)
Pages (from-to)	9-17
Number of pages	9
Journal	FEMS Microbiology Letters
Volume	237
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2004.06.008
State	Published - Aug 1 2004

Keywords

Francisella
Genome
Polymorphism

ASJC Scopus subject areas

General Medicine

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10.1016/j.femsle.2004.06.008

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Samrakandi, M. M., Zhang, C., Zhang, M., Nietfeldt, J., Kim, J., Iwen, P. C., Olson, M. E., Fey, P. D., Duhamel, G. E., Hinrichs, S. H., Cirillo, J. D., & Benson, A. K. (2004). Genome diversity among regional populations of Francisella tularensis subspecies tularensis and Francisella tularensis subspecies holarctica isolated from the US. FEMS Microbiology Letters, 237(1), 9-17. https://doi.org/10.1016/j.femsle.2004.06.008

Samrakandi, MM, Zhang, C, Zhang, M, Nietfeldt, J, Kim, J, Iwen, PC, Olson, ME, Fey, PD, Duhamel, GE, Hinrichs, SH, Cirillo, JD & Benson, AK 2004, 'Genome diversity among regional populations of Francisella tularensis subspecies tularensis and Francisella tularensis subspecies holarctica isolated from the US', FEMS Microbiology Letters, vol. 237, no. 1, pp. 9-17. https://doi.org/10.1016/j.femsle.2004.06.008

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abstract = "Francisella tularensis is a highly infectious facultative intracellular pathogen that is considered a potential agent of bioterrorism. Four different F. tularensis subspecies have been identified and they appear to display different ecological and virulence characteristics as well as differences in geographical distribution. One simple explanation for the variation in ecological and virulence characteristics is that they are conferred by differences in genome content. To characterize genome content among stains isolated from United States, we have used a DNA microarray designed from a shotgun library of a reference strain. Polymorphisms distributed among polyphyletic sets of strains was the most common pattern of genome alteration observed, indicating that strain-specific genome variability is significant. Nonetheless, 13 different contiguous segments of the genome were found to be missing exclusively in each of the subsp. holarctica strains tested. All 13 are associated with repeat sequences or transposases that could promote insertion/deletion events. Comparison of the live vaccine strain to other holarctica strains also identified three regions that are absent exclusively in the live vaccine strain derived from holarctica.",

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note = "Funding Information: We thank Karen W. Post, Kenneth Mills, Rebecca J. Morton, and S. Hanover for contribution of strains used in our studies. We thank Suat Cirillo and Rhonda Noel for technical assistance. We greatly appreciate the willingness of members of the Francisella genome sequencing project ( http://artedi.ebc.uu.se/Projects/Francisella/ ) to provide access to their unpublished partial genome sequence data. This project was supported in part by University of Nebraska Vice Chancellor's Collaborative Development fund and the University of Nebraska Center for Biosecurity.",

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AU - Nietfeldt, J.

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AU - Iwen, P. C.

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AU - Duhamel, G. E.

AU - Hinrichs, S. H.

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AU - Benson, A. K.

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N2 - Francisella tularensis is a highly infectious facultative intracellular pathogen that is considered a potential agent of bioterrorism. Four different F. tularensis subspecies have been identified and they appear to display different ecological and virulence characteristics as well as differences in geographical distribution. One simple explanation for the variation in ecological and virulence characteristics is that they are conferred by differences in genome content. To characterize genome content among stains isolated from United States, we have used a DNA microarray designed from a shotgun library of a reference strain. Polymorphisms distributed among polyphyletic sets of strains was the most common pattern of genome alteration observed, indicating that strain-specific genome variability is significant. Nonetheless, 13 different contiguous segments of the genome were found to be missing exclusively in each of the subsp. holarctica strains tested. All 13 are associated with repeat sequences or transposases that could promote insertion/deletion events. Comparison of the live vaccine strain to other holarctica strains also identified three regions that are absent exclusively in the live vaccine strain derived from holarctica.

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Genome diversity among regional populations of Francisella tularensis subspecies tularensis and Francisella tularensis subspecies holarctica isolated from the US

Abstract

Keywords

ASJC Scopus subject areas

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