Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4

Benjamin Janto, Azad Ahmed, Masahiro Ito, Jun Liu, David B. Hicks, Sarah Pagni, Oliver J. Fackelmayer, Terry Ann Smith, Joshua Earl, Liam D.H. Elbourne, Karl Hassan, Ian T. Paulsen, Anne Brit Kolstø, Nicolas J. Tourasse, Garth D. Ehrlich, Robert Boissy, D. Mack Ivey, Gang Li, Yanfen Xue, Yanhe MaFen Z. Hu, Terry A. Krulwich

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH>9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B. pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.

Original languageEnglish (US)
Pages (from-to)3289-3309
Number of pages21
JournalEnvironmental microbiology
Volume13
Issue number12
DOIs
StatePublished - Dec 2011

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Janto, B., Ahmed, A., Ito, M., Liu, J., Hicks, D. B., Pagni, S., Fackelmayer, O. J., Smith, T. A., Earl, J., Elbourne, L. D. H., Hassan, K., Paulsen, I. T., Kolstø, A. B., Tourasse, N. J., Ehrlich, G. D., Boissy, R., Ivey, D. M., Li, G., Xue, Y., ... Krulwich, T. A. (2011). Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4. Environmental microbiology, 13(12), 3289-3309. https://doi.org/10.1111/j.1462-2920.2011.02591.x