Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

Blake T. Hovde, Erik R. Hanschen, Christina R. Steadman Tyler, Chien Chi Lo, Yuliya Kunde, Karen Davenport, Hajnalka Daligault, Joseph Msanne, Stephanie Canny, Seong il Eyun, Jean Jack M. Riethoven, Juergen Polle, Shawn R. Starkenburg

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Selection of highly productive algal strains is crucial for establishing economically viable biomass and bioproduct cultivation systems. Characterization of algal genomes, including understanding strain-specific differences in genome content and architecture is a critical step in this process. Using genomic analyses, we demonstrate significant differences between three strains of Chlorella sorokiniana (strain 1228, UTEX 1230, and DOE1412). We found that unique, strain-specific genes comprise a substantial proportion of each genome, and genomic regions with >80% local nucleotide identity constitute <15% of each genome among the strains, indicating substantial strain specific evolution. Furthermore, cataloging of meiosis and other sex-related genes in C. sorokiniana strains suggests strategic breeding could be utilized to improve biomass and bioproduct yields if a sexual cycle can be characterized. Finally, preliminary investigation of epigenetic machinery suggests the presence of potentially unique transcriptional regulation in each strain. Our data demonstrate that these three C. sorokiniana strains represent significantly different genomic content. Based on these findings, we propose individualized assessment of each strain for potential performance in cultivation systems.

Original languageEnglish (US)
Pages (from-to)449-461
Number of pages13
JournalAlgal Research
Volume35
DOIs
StatePublished - Nov 2018

Keywords

  • Chlorella sorokiniana
  • DNA methylation
  • Genome analysis
  • Meiosis
  • Polyketide synthetase (PKS)

ASJC Scopus subject areas

  • Agronomy and Crop Science

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  • Cite this

    Hovde, B. T., Hanschen, E. R., Steadman Tyler, C. R., Lo, C. C., Kunde, Y., Davenport, K., Daligault, H., Msanne, J., Canny, S., Eyun, S. I., Riethoven, J. J. M., Polle, J., & Starkenburg, S. R. (2018). Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae). Algal Research, 35, 449-461. https://doi.org/10.1016/j.algal.2018.09.012