TY - JOUR
T1 - Genomic mining type III secretion system effectors in Pseudomonas syringae yields new picks for all TTSS prospectors
AU - Collmer, Alan
AU - Lindeberg, Magdalen
AU - Petnicki-Ocwieja, Tanja
AU - Schneider, David J.
AU - Alfano, James R.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Many bacterial pathogens of plants and animals use a type III secretion system (TTSS) to deliver virulence effector proteins into host cells. Because effectors are heterogeneous in sequence and function, there has not been a systematic way to identify the genes encoding them in pathogen genomes, and our current inventories are probably incomplete. A pre-closure draft sequence of Pseudomonas syringae pv. tomato DC3000, a pathogen of tomato and Arabidopsis, has recently supported five complementary studies which, collectively, identify 36 TTSS-secreted proteins and many more candidate effectors in this strain. These studies demonstrate the advantages of combining experimental and computational approaches, and they yield new insights into TTSS effectors and virulence regulation in P. syringae, potential effector targeting signals in all TTSS-dependent pathogens, and strategies for finding TTSS effectors in other bacteria that have sequenced genomes.
AB - Many bacterial pathogens of plants and animals use a type III secretion system (TTSS) to deliver virulence effector proteins into host cells. Because effectors are heterogeneous in sequence and function, there has not been a systematic way to identify the genes encoding them in pathogen genomes, and our current inventories are probably incomplete. A pre-closure draft sequence of Pseudomonas syringae pv. tomato DC3000, a pathogen of tomato and Arabidopsis, has recently supported five complementary studies which, collectively, identify 36 TTSS-secreted proteins and many more candidate effectors in this strain. These studies demonstrate the advantages of combining experimental and computational approaches, and they yield new insights into TTSS effectors and virulence regulation in P. syringae, potential effector targeting signals in all TTSS-dependent pathogens, and strategies for finding TTSS effectors in other bacteria that have sequenced genomes.
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U2 - 10.1016/S0966-842X(02)02451-4
DO - 10.1016/S0966-842X(02)02451-4
M3 - Review article
C2 - 12377556
AN - SCOPUS:0036791896
VL - 10
SP - 462
EP - 469
JO - Trends in Microbiology
JF - Trends in Microbiology
SN - 0966-842X
IS - 10
ER -