TY - JOUR
T1 - Genomic organization of MUC4 mucin gene
T2 - Towards the characterization of splice variants
AU - Escande, Fabienne
AU - Lemaitre, Laurent
AU - Moniaux, Nicolas
AU - Batra, Surinder K.
AU - Aubert, Jean Pierre
AU - Buisine, Marie Pierre
PY - 2002
Y1 - 2002
N2 - The human MUC4 gene encodes a large membrane-associated mucin, characterized by a mucin tandem repeat domain and a growth factor-like transmembrane domain. In addition to the originally published sequence (sv0-MUC4), several MUC4 cDNA sequences (called sv1-MUC4 to sv21-MUC4, MUC4/X, MUC4/Y) from various tissues and cell lines have been recently described. They differ from sv0-MUC4 by deletions and/or insertions located in the 3′ region or, for two of them, by deletion of the central repetitive domain. To establish the nature of the mechanisms responsible for the diversity of MUC4 transcripts, the genomic structure of the 3′ region of the human MUC4 gene was determined. Our results show that it spans approximately 30.8 kb of genomic DNA and is composed of 24 exons, including one alternative exon which was exclusively reported for sv1-MUC4. Moreover, we have shown that the di(erent MUC4 transcripts are generated by several mechanisms, including the alternative use of cassette exons, exon skipping or use of cryptic splice donor/acceptor sites.
AB - The human MUC4 gene encodes a large membrane-associated mucin, characterized by a mucin tandem repeat domain and a growth factor-like transmembrane domain. In addition to the originally published sequence (sv0-MUC4), several MUC4 cDNA sequences (called sv1-MUC4 to sv21-MUC4, MUC4/X, MUC4/Y) from various tissues and cell lines have been recently described. They differ from sv0-MUC4 by deletions and/or insertions located in the 3′ region or, for two of them, by deletion of the central repetitive domain. To establish the nature of the mechanisms responsible for the diversity of MUC4 transcripts, the genomic structure of the 3′ region of the human MUC4 gene was determined. Our results show that it spans approximately 30.8 kb of genomic DNA and is composed of 24 exons, including one alternative exon which was exclusively reported for sv1-MUC4. Moreover, we have shown that the di(erent MUC4 transcripts are generated by several mechanisms, including the alternative use of cassette exons, exon skipping or use of cryptic splice donor/acceptor sites.
KW - Alternative splicing
KW - MUC4
KW - Membrane-associated
KW - Mucin
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U2 - 10.1046/j.1432-1033.2002.03032.x
DO - 10.1046/j.1432-1033.2002.03032.x
M3 - Article
C2 - 12153560
AN - SCOPUS:0036365471
SN - 0014-2956
VL - 269
SP - 3637
EP - 3644
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 15
ER -