Genotoxic effects of tobacco extract on Chinese hamster ovary cells

Amit H. Trivedi; Bhavana J. Dave; Siddharth G. Adhvaryu

doi:10.1016/0304-3835(93)90081-J

Genotoxic effects of tobacco extract on Chinese hamster ovary cells

Amit H. Trivedi, Bhavana J. Dave, Siddharth G. Adhvaryu

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Genotoxic effects of an aqueous extract of Nicotiana tabacum, a variety commonly used in India for chewing purposes, were analysed on CHO cells utilizing two different cytogenetic end-points, namely, chromosome aberration frequency and sister chromatid exchange frequency. Statistically significant elevations in the values of both the markers clearly indicated chromosome damaging effects of the extract. Elevations in chromosome aberration and sister chromatid exchange frequencies are suggestive of intrastrand and interstrand DNA cross-links following exposure to tobacco. The effects observed following treatment with low dose for longer duration are of relevance to the condition of the oral mucosa of the chronic smokeless tobacco users.

Original language	English (US)
Pages (from-to)	107-112
Number of pages	6
Journal	Cancer Letters
Volume	70
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3835(93)90081-J
State	Published - Jun 15 1993
Externally published	Yes

Keywords

Chinese hamster ovary cells
aqueous extract
chromosome aberration
genotoxicity
sister chromatid exchange
tobacco

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(93)90081-J

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title = "Genotoxic effects of tobacco extract on Chinese hamster ovary cells",

abstract = "Genotoxic effects of an aqueous extract of Nicotiana tabacum, a variety commonly used in India for chewing purposes, were analysed on CHO cells utilizing two different cytogenetic end-points, namely, chromosome aberration frequency and sister chromatid exchange frequency. Statistically significant elevations in the values of both the markers clearly indicated chromosome damaging effects of the extract. Elevations in chromosome aberration and sister chromatid exchange frequencies are suggestive of intrastrand and interstrand DNA cross-links following exposure to tobacco. The effects observed following treatment with low dose for longer duration are of relevance to the condition of the oral mucosa of the chronic smokeless tobacco users.",

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T1 - Genotoxic effects of tobacco extract on Chinese hamster ovary cells

AU - Trivedi, Amit H.

AU - Dave, Bhavana J.

AU - Adhvaryu, Siddharth G.

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N2 - Genotoxic effects of an aqueous extract of Nicotiana tabacum, a variety commonly used in India for chewing purposes, were analysed on CHO cells utilizing two different cytogenetic end-points, namely, chromosome aberration frequency and sister chromatid exchange frequency. Statistically significant elevations in the values of both the markers clearly indicated chromosome damaging effects of the extract. Elevations in chromosome aberration and sister chromatid exchange frequencies are suggestive of intrastrand and interstrand DNA cross-links following exposure to tobacco. The effects observed following treatment with low dose for longer duration are of relevance to the condition of the oral mucosa of the chronic smokeless tobacco users.

AB - Genotoxic effects of an aqueous extract of Nicotiana tabacum, a variety commonly used in India for chewing purposes, were analysed on CHO cells utilizing two different cytogenetic end-points, namely, chromosome aberration frequency and sister chromatid exchange frequency. Statistically significant elevations in the values of both the markers clearly indicated chromosome damaging effects of the extract. Elevations in chromosome aberration and sister chromatid exchange frequencies are suggestive of intrastrand and interstrand DNA cross-links following exposure to tobacco. The effects observed following treatment with low dose for longer duration are of relevance to the condition of the oral mucosa of the chronic smokeless tobacco users.

KW - Chinese hamster ovary cells

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KW - chromosome aberration

KW - genotoxicity

KW - sister chromatid exchange

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Genotoxic effects of tobacco extract on Chinese hamster ovary cells

Abstract

Keywords

ASJC Scopus subject areas

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