Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains

Kim Ziebell, Marina Steele, Yongxiang Zhang, Andrew Benson, Eduardo N. Taboada, Chad Laing, Scott McEwen, Bruce Ciebin, Roger Johnson, Victor Gannon

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40 Scopus citations

Abstract

In this study, the association between genotypic and selected phenotypic characteristics was examined in a collection of Canadian Escherichia coli O157:H7 strains isolated from humans and cattle in the provinces of Alberta, Ontario, Saskatchewan, and Quebec. In a subset of 69 strains selected on the basis of specific phage types (PTs), a strong correlation between the lineage-specific polymorphism assay (LSPA6) genotype and PT was observed with all strains of PTs 4, 14, 21, 31, 33, and 87 belonging to the LSPA6 lineage I (LSPA6-LI) genotype, while those of PTs 23, 45, 67, and 74 belonged to LSPA6 lineage II (LSPA6-LII) genotypes. This correlation was maintained when additional strains of each PT were tested. E. coli O157:H7 strains with the LSPA6-LI genotype were much more common in the collection than were the LSPA6-LII or lineage I/II (LSPA6-LI/II)-related genotypes (82.6, 11.2, and 5.8%, respectively). Of the strains tested, proportionately more LSPA6-LI than LSPA6-LII genotype strains were isolated from humans (52.7% versus 19.7%) than from cattle (47.8% versus 80.2%). In addition, 96.7% of the LSPA6-LII strains carried the stx2c variant gene, while only 50.0% of LSPA6-LI/II and 2.7% of LSPA6-LI strains carried this gene. LSPA6-LII strains were also significantly more likely to possess the colicin D gene, cda (50.8% versus 23.2%), and have combined resistance to streptomycin, sulfisoxazole, and tetracycline (72.1% versus 0.9%) than were LSPA6-LI strains. The LSPA6 genotype- and PT-related characteristics identified may be important markers of specific ecotypes of E. coli O157:H7 that have unique epidemiological and virulence characteristics.

Original languageEnglish (US)
Pages (from-to)4314-4323
Number of pages10
JournalApplied and environmental microbiology
Volume74
Issue number14
DOIs
StatePublished - Jul 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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    Ziebell, K., Steele, M., Zhang, Y., Benson, A., Taboada, E. N., Laing, C., McEwen, S., Ciebin, B., Johnson, R., & Gannon, V. (2008). Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains. Applied and environmental microbiology, 74(14), 4314-4323. https://doi.org/10.1128/AEM.02821-07