Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption

R. Nandakumar, A. M. Gounot, B. Mattiasson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A procedure for the enhanced lysis of mucous producing psychrotrophic Gram positive bacteria for subsequent enzyme studies is described. An initial washing of bacterial cells with Tween 80 was found to improve the degree of cell disruption in subsequent sonication or grinding with glass beads, resulting in about 20-200% increase in total soluble protein content. However, in terms of lactate dehydrogenase (LDH) activity present in the lysate, pretreatment with Tween 80 was more effective in combination with grinding, especially in the highly mucous producing strain GY11. The type of surfactant used in the pretreatment procedure before grinding strongly influenced the percentage lysis of tested strains, both in terms of released soluble protein and enzyme activity. Zymograms of LDH and glutamate dehydrogenase (GDH) activity present in the lysates also very well supported the results obtained by total protein and enzyme activity measurements. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Biotechnology
Volume83
Issue number3
DOIs
StatePublished - Oct 13 2000

Keywords

  • Cell lysis
  • Psychrotrophic bacteria
  • Surfactants

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption'. Together they form a unique fingerprint.

  • Cite this