Giardia spp. Distribution of contractile proteins in the attachment organelle

Dennis E. Feely, Judy V. Schollmeyer, Stanley L. Erlandsen

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Giardia spp. trophozoites isolated from rat small intestine were examined by light microscopy, electron microscopy, SDS-gel electrophoresis, and immunocytochemistry. In SDS-gels of protein extracts of isolated Giardia spp. trophozoites protein bands corresponding to myosin, α-actinin, and actin were identified by comigration with avian myofibril proteins and molecular weight standards. Actin was specifically identified in SDS-gels by immunoautoradiography. Immunostaining for actin, α-actinin, myosin, and tropomyosin in trophozoites was demonstrated in the periphery of the ventral disc in an area corresponding to the lateral crest. Electron-dense fibrillar was observed in the lateral crest of the ventral disc by electron microscopy. Immunostaining for actin and α-actinin was also observed in the area of the median body, a microtubular organelle, and in electron-dense fibrillar material associated with the intracellular axonemes of the posterior-lateral flagella. The localization of these contractile proteins in the ventral disc suggests that they may play an important role in the mechanism of trophozoite attachment.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalExperimental Parasitology
Volume53
Issue number1
DOIs
StatePublished - Feb 1982

Keywords

  • Attachment organelle
  • Biochemistry
  • Giardia duodenalis
  • Giardia muris
  • Immunochemistry
  • Morphology
  • Proteins, contractile
  • Protozoa, parasitic

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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