Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages

Z. G. Jiang; C. Piggee; M. P. Heyes; C. Murphy; B. Quearry; M. Bauer; J. Zheng; H. E. Gendelman; S. p. Markey

doi:10.1016/S0165-5728(01)00315-0

Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages

Z. G. Jiang, C. Piggee, M. P. Heyes, C. Murphy, B. Quearry, M. Bauer, J. Zheng, H. E. Gendelman, S. p. Markey

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

We sought to identify neurotoxin(s) secreted by HIV-1-infected mononuclear phagocytes that could contribute to the pathophysiology of HIV-1-associated dementia (HAD). Neurotoxic factors were characterized in batches of conditioned media (CM) from human monocyte-derived macrophages (MDM) infected with HIV-1_ADA and/or activated with lipopolysaccharide (LPS). All of the neurotoxicity was: present in the < 3000-Da fraction; blocked by 5 μM MK801; and not trypsin sensitive or extractable into polar organic solvents. Glutamate measured in CM accounted for all neurotoxic effects observed from HIV/LPS CM in astrocyte-poor neuronal cultures and may contribute to the pathophysiology of HIV-1-associated dementia.

Original language	English (US)
Pages (from-to)	97-107
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	117
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(01)00315-0
State	Published - Jul 2 2001

Keywords

Dementia
Lipopolysaccharide
Mononuclear phagocyte
NMDA
Neurodegeneration

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

Access to Document

10.1016/S0165-5728(01)00315-0

Cite this

@article{f5d5f6dfd5d04b02a9292798349cb0ef,

title = "Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages",

abstract = "We sought to identify neurotoxin(s) secreted by HIV-1-infected mononuclear phagocytes that could contribute to the pathophysiology of HIV-1-associated dementia (HAD). Neurotoxic factors were characterized in batches of conditioned media (CM) from human monocyte-derived macrophages (MDM) infected with HIV-1ADA and/or activated with lipopolysaccharide (LPS). All of the neurotoxicity was: present in the < 3000-Da fraction; blocked by 5 μM MK801; and not trypsin sensitive or extractable into polar organic solvents. Glutamate measured in CM accounted for all neurotoxic effects observed from HIV/LPS CM in astrocyte-poor neuronal cultures and may contribute to the pathophysiology of HIV-1-associated dementia.",

keywords = "Dementia, Lipopolysaccharide, Mononuclear phagocyte, NMDA, Neurodegeneration",

author = "Jiang, {Z. G.} and C. Piggee and Heyes, {M. P.} and C. Murphy and B. Quearry and M. Bauer and J. Zheng and Gendelman, {H. E.} and Markey, {S. p.}",

note = "Funding Information: This study was supported by NIH funding through 1Z01-MH00279 and 2 R01 NS34239-04A1.",

year = "2001",

month = jul,

day = "2",

doi = "10.1016/S0165-5728(01)00315-0",

language = "English (US)",

volume = "117",

pages = "97--107",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages

AU - Jiang, Z. G.

AU - Piggee, C.

AU - Heyes, M. P.

AU - Murphy, C.

AU - Quearry, B.

AU - Bauer, M.

AU - Zheng, J.

AU - Gendelman, H. E.

AU - Markey, S. p.

N1 - Funding Information: This study was supported by NIH funding through 1Z01-MH00279 and 2 R01 NS34239-04A1.

PY - 2001/7/2

Y1 - 2001/7/2

N2 - We sought to identify neurotoxin(s) secreted by HIV-1-infected mononuclear phagocytes that could contribute to the pathophysiology of HIV-1-associated dementia (HAD). Neurotoxic factors were characterized in batches of conditioned media (CM) from human monocyte-derived macrophages (MDM) infected with HIV-1ADA and/or activated with lipopolysaccharide (LPS). All of the neurotoxicity was: present in the < 3000-Da fraction; blocked by 5 μM MK801; and not trypsin sensitive or extractable into polar organic solvents. Glutamate measured in CM accounted for all neurotoxic effects observed from HIV/LPS CM in astrocyte-poor neuronal cultures and may contribute to the pathophysiology of HIV-1-associated dementia.

AB - We sought to identify neurotoxin(s) secreted by HIV-1-infected mononuclear phagocytes that could contribute to the pathophysiology of HIV-1-associated dementia (HAD). Neurotoxic factors were characterized in batches of conditioned media (CM) from human monocyte-derived macrophages (MDM) infected with HIV-1ADA and/or activated with lipopolysaccharide (LPS). All of the neurotoxicity was: present in the < 3000-Da fraction; blocked by 5 μM MK801; and not trypsin sensitive or extractable into polar organic solvents. Glutamate measured in CM accounted for all neurotoxic effects observed from HIV/LPS CM in astrocyte-poor neuronal cultures and may contribute to the pathophysiology of HIV-1-associated dementia.

KW - Dementia

KW - Lipopolysaccharide

KW - Mononuclear phagocyte

KW - NMDA

KW - Neurodegeneration

UR - http://www.scopus.com/inward/record.url?scp=0035796893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035796893&partnerID=8YFLogxK

U2 - 10.1016/S0165-5728(01)00315-0

DO - 10.1016/S0165-5728(01)00315-0

M3 - Article

C2 - 11431009

AN - SCOPUS:0035796893

SN - 0165-5728

VL - 117

SP - 97

EP - 107

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this