Glycolytic enzymes can modulate cellular life span

Hiroshi Kondoh; Matilde E. Lleonart; Jesus Gil; Jing Wang; Paolo Degan; Gordon Peters; Dolores Martinez; Amancio Carnero; David Beach

Glycolytic enzymes can modulate cellular life span

Hiroshi Kondoh, Matilde E. Lleonart, Jesus Gil, Jing Wang, Paolo Degan, Gordon Peters, Dolores Martinez, Amancio Carnero, David Beach

Eppley Institute for Cancer Research

Research output: Contribution to journal › Article

454 Scopus citations

Abstract

An unbiased screen for genes that can immortalize mouse embryonic fibroblasts identified the glycolytic enzyme phosphoglycerate mutase (PGM). A 2-fold increase in PGM activity enhances glycolytic flux, allows indefinite proliferation, and renders cells resistant to ras-induced arrest. Glucosephosphate isomerase, another glycolytic enzyme, displays similar activity and, conversely, depletion of PGM or glucosephosphate isomerase with short interfering RNA triggers premature senescence. Immortalized mouse embryonic fibroblasts and mouse embryonic stem cells display higher glycolytic flux and more resistance to oxidative damage than senescent cells. Because wild-type p53 down-regulates PGM, mutation of p53 can facilitate immortalization via effects on PGM levels and glycolysis.

Original language	English (US)
Pages (from-to)	177-185
Number of pages	9
Journal	Cancer Research
Volume	65
Issue number	1
State	Published - Jan 1 2005

ASJC Scopus subject areas

Oncology
Cancer Research

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Kondoh, H., Lleonart, M. E., Gil, J., Wang, J., Degan, P., Peters, G., Martinez, D., Carnero, A., & Beach, D. (2005). Glycolytic enzymes can modulate cellular life span. Cancer Research, 65(1), 177-185.

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AU - Kondoh, Hiroshi

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AU - Gil, Jesus

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AU - Peters, Gordon

AU - Martinez, Dolores

AU - Carnero, Amancio

AU - Beach, David

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