Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines

John MeKibbin; Laurie Arcolano; Karl Anders Karlsson; Goran Larson; Jan Thurin; Michael Brattain

doi:10.1016/0005-2760(88)90182-8

Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines

John MeKibbin, Laurie Arcolano, Karl Anders Karlsson, Goran Larson, Jan Thurin, Michael Brattain

Eppley Institute for Cancer Research

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides G_M2 and 6_M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.

Original language	English (US)
Pages (from-to)	235-246
Number of pages	12
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	958
Issue number	2
DOIs	https://doi.org/10.1016/0005-2760(88)90182-8
State	Published - Feb 4 1988

Keywords

(Human colon carcinoma cell)
Antitumor drug
Drug resistance
G
G
Gal α1 → 4Galβ1 → 4Glc → ceramide
Gal αl → 4Gal → ceramide
GalNAβ1 → 4[NeuAcα2 → 3]Galβ1 → 4G1 → ceramide
GalNacβ1 → 3Galα1 → 4Galβ1 → 4Glc → ceramide
Galβ1 → 3GalNAcβ1 → 4[NeuAcα2 → 3]Galβ1 → 4Glc → ceramide
Galβ1 → 3[Fucα1 → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
Galβ1 → 4Glc → ceramide
Galβ1 → 4GlcNAcβl → 3Galβ1 → 4Glc → ceramide
Galβ1 → 4[Fuc αl → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
Glycosphingolipid
HPTLC
Lewis a fucolipid
Lewis a isomer or 'X hapten'
NeuAc α2 → 8NeuAc α2 → 3Galβ1 → 4GlcNAcβl→ 3Galβ1→ 4Glc→ ceramide
NeuAca2 → 3Galβ1 → 4[Fucα1 → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
NeuAcα2 → 3Galβ1 → 3[Fucαl → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
NeuAcα2 → 3Galβ1 → 4Glc → ceramide
NeuAcα2 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc → ceramide
TLC
disialosyl paragloboside
g
g
galabiosylceramide
globoside
globotriaosyl ceramide
high-perofrmance thin-layer chromatography
lactosyl ceramide
paragloboside
sialosyl 'X'
sialosyl Lewis a
sialosyl paragloboside
thin-layer chromatography

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

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Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines. / MeKibbin, John; Arcolano, Laurie; Karlsson, Karl Anders; Larson, Goran; Thurin, Jan; Brattain, Michael.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 958, No. 2, 04.02.1988, p. 235-246.

Research output: Contribution to journal › Article › peer-review

@article{d77aa56d29404ec285a9787a12f064fb,

title = "Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines",

abstract = "Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides GM2 and 6M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.",

keywords = "(Human colon carcinoma cell), Antitumor drug, Drug resistance, G, G, Gal α1 → 4Galβ1 → 4Glc → ceramide, Gal αl → 4Gal → ceramide, GalNAβ1 → 4[NeuAcα2 → 3]Galβ1 → 4G1 → ceramide, GalNacβ1 → 3Galα1 → 4Galβ1 → 4Glc → ceramide, Galβ1 → 3GalNAcβ1 → 4[NeuAcα2 → 3]Galβ1 → 4Glc → ceramide, Galβ1 → 3[Fucα1 → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide, Galβ1 → 4Glc → ceramide, Galβ1 → 4GlcNAcβl → 3Galβ1 → 4Glc → ceramide, Galβ1 → 4[Fuc αl → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide, Glycosphingolipid, HPTLC, Lewis a fucolipid, Lewis a isomer or 'X hapten', NeuAc α2 → 8NeuAc α2 → 3Galβ1 → 4GlcNAcβl→ 3Galβ1→ 4Glc→ ceramide, NeuAca2 → 3Galβ1 → 4[Fucα1 → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide, NeuAcα2 → 3Galβ1 → 3[Fucαl → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide, NeuAcα2 → 3Galβ1 → 4Glc → ceramide, NeuAcα2 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide, NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc → ceramide, TLC, disialosyl paragloboside, g, g, galabiosylceramide, globoside, globotriaosyl ceramide, high-perofrmance thin-layer chromatography, lactosyl ceramide, paragloboside, sialosyl 'X', sialosyl Lewis a, sialosyl paragloboside, thin-layer chromatography",

author = "John MeKibbin and Laurie Arcolano and Karlsson, {Karl Anders} and Goran Larson and Jan Thurin and Michael Brattain",

note = "Funding Information: This work was supported by National Institutes of Health Grant CA-29495, 40449 and Swedish Medical Research Grant 3967. Human tissues were provided through the University of Alabama in Birmingham - Veterans Administration Medical Center Tissue Procurement Facility, Birmingham, AL.",

year = "1988",

month = feb,

day = "4",

doi = "10.1016/0005-2760(88)90182-8",

language = "English (US)",

volume = "958",

pages = "235--246",

journal = "BBA - Specialised Section On Lipids and Related Subjects",

issn = "1388-1981",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines

AU - MeKibbin, John

AU - Arcolano, Laurie

AU - Karlsson, Karl Anders

AU - Larson, Goran

AU - Thurin, Jan

AU - Brattain, Michael

N1 - Funding Information: This work was supported by National Institutes of Health Grant CA-29495, 40449 and Swedish Medical Research Grant 3967. Human tissues were provided through the University of Alabama in Birmingham - Veterans Administration Medical Center Tissue Procurement Facility, Birmingham, AL.

PY - 1988/2/4

Y1 - 1988/2/4

N2 - Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides GM2 and 6M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.

AB - Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides GM2 and 6M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.

KW - (Human colon carcinoma cell)

KW - Antitumor drug

KW - Drug resistance

KW - G

KW - Gal α1 → 4Galβ1 → 4Glc → ceramide

KW - Gal αl → 4Gal → ceramide

KW - GalNAβ1 → 4[NeuAcα2 → 3]Galβ1 → 4G1 → ceramide

KW - GalNacβ1 → 3Galα1 → 4Galβ1 → 4Glc → ceramide

KW - Galβ1 → 3GalNAcβ1 → 4[NeuAcα2 → 3]Galβ1 → 4Glc → ceramide

KW - Galβ1 → 3[Fucα1 → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - Galβ1 → 4Glc → ceramide

KW - Galβ1 → 4GlcNAcβl → 3Galβ1 → 4Glc → ceramide

KW - Galβ1 → 4[Fuc αl → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - Glycosphingolipid

KW - HPTLC

KW - Lewis a fucolipid

KW - Lewis a isomer or 'X hapten'

KW - NeuAc α2 → 8NeuAc α2 → 3Galβ1 → 4GlcNAcβl→ 3Galβ1→ 4Glc→ ceramide

KW - NeuAca2 → 3Galβ1 → 4[Fucα1 → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 3Galβ1 → 3[Fucαl → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc → ceramide

KW - TLC

KW - disialosyl paragloboside

KW - g

KW - galabiosylceramide

KW - globoside

KW - globotriaosyl ceramide

KW - high-perofrmance thin-layer chromatography

KW - lactosyl ceramide

KW - paragloboside

KW - sialosyl 'X'

KW - sialosyl Lewis a

KW - sialosyl paragloboside

KW - thin-layer chromatography

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U2 - 10.1016/0005-2760(88)90182-8

DO - 10.1016/0005-2760(88)90182-8

M3 - Article

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AN - SCOPUS:0023848316

VL - 958

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EP - 246

JO - BBA - Specialised Section On Lipids and Related Subjects

JF - BBA - Specialised Section On Lipids and Related Subjects

SN - 1388-1981

IS - 2

ER -

Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines

Abstract

Keywords

ASJC Scopus subject areas

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