Glycosylation potential of human prostate cancer cell lines

Yin Gao, Vishwanath B. Chachadi, Pi Wan Cheng, Inka Brockhausen

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Altered glycosylation is a universal feature of cancer cells and altered glycans can help cancer cells escape immune surveillance, facilitate tumor invasion, and increase malignancy. The goal of this study was to identify specific glycoenzymes, which could distinguish prostate cancer cells from normal prostatic cells. We investigated enzymatic activities and gene expression levels of key glycosyl-And sulfotransferases responsible for the assembly of O-And N-glycans in several prostatic cells. These cells included immortalized RWPE-1 cells derived from normal prostatic tissues, and prostate cancer cells derived from metastasis in bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP). We found that all cells were capable of synthesizing complex N-glycans and O-glycans with the core 1 structure, and each cell line had characteristic biosynthetic pathways to modify these structures. The in vitro measured activities corresponded well to the mRNA levels of glycosyltransferases and sulfotransferases. Lectin and antibody binding to whole cells supported these results, which form the basis for the development of tumor cellspecific targeting strategies.

Original languageEnglish (US)
Pages (from-to)525-537
Number of pages13
JournalGlycoconjugate Journal
Volume29
Issue number7
DOIs
StatePublished - Oct 2012

Keywords

  • Glycosyltransferase activities
  • N-glycosylation
  • O-glycosylation
  • Prostate cancer cells
  • Real-Time PCR

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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