Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation

H. Zhou, J. W. Arrowsmith, M. E. Fromm, C. M. Hironaka, M. L. Taylor, D. Rodriguez, M. E. Pajeau, S. M. Brown, C. G. Santino, J. E. Fry

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9-12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalPlant Cell Reports
Volume15
Issue number3-4
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

Keywords

  • CP4/GOX
  • Wheat
  • embryos
  • glyphosate
  • transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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    Zhou, H., Arrowsmith, J. W., Fromm, M. E., Hironaka, C. M., Taylor, M. L., Rodriguez, D., Pajeau, M. E., Brown, S. M., Santino, C. G., & Fry, J. E. (1995). Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Reports, 15(3-4), 159-163. https://doi.org/10.1007/BF00193711