GM-CSF-mobilized peripheral blood CD34+ cells differ from steady-state bone marrow CD34+ cells in adhesion molecule expression

T. Watanabe, B. Dave, D. G. Heimann, E. Lethaby, A. Kessinger, J. E. Talmadge

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

To determine the effect of growth factor mobilization on the expression of adhesion molecules, we compared CD34+ progenitor cell (PC) populations from steady-state bone marrow (BM) with granulocyte-macrophage colony-stimulating factor (GM-CSF)-mobilized apheresis products (peripheral blood stem cell (PSC)) using flow cytometry. To increase the accuracy of this analysis, CD34+ cells were enriched (MiniMACS) before cytometric analysis. A significantly lower expression of very late antigen-4 (VLA-4), leukocyte function antigen-1 (LFA-1) and LFA-3 were observed on PSC compared to BM CD34+ cells. In addition, significantly lower mean fluorescence intensity (MFI) of VLA-4, VLA-5, intercellular adhesion molecule-1 (ICAM-1), and sialyl Lewis(x) were observed on PSC as compared to BM CD34+ cells. Significantly higher levels of L-selectin and CD44 expression were observed on PSC as compared to BM CD34+ cells based on frequency and MFI (P ≤ 0.05). In addition, the duration of GM-CSF administration or number of prior aphereses had no effect on adhesion molecule expression. These data suggest that decreased expression of adhesion molecules including VLA-4, LFA-1, ICAM-1 and LFA-3 play a role in PC mobilization. Based on these studies, we suggest that PC mobilization occurs as a stochastic process and is associated with the selection of CD34+ cells with low adhesion molecule expression.

Original languageEnglish (US)
Pages (from-to)1175-1181
Number of pages7
JournalBone marrow transplantation
Volume19
Issue number12
DOIs
StatePublished - Jun 2 1997

Keywords

  • Adhesion molecule
  • Bone marrow CD34 cells
  • GM-CSF mobilization
  • Peripheral blood CD34 cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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