Gradient temperature hybridization using a thermocycler for RNase protection assays

Luwen Zhang

doi:10.1385/mb:14:1:73

Gradient temperature hybridization using a thermocycler for RNase protection assays

Luwen Zhang

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The RNase protection assay (RPA) is a sensitive and quantitative assay for the detection of specific RNAs. Because some probes require an optimal temperature for efficient hybridization to the target RNAs, preliminary experiments to determine the optimal temperatures have to be done empirically, a process that is laborious and time-consuming. In order to simplify this process, a new gradient temperature hybridization procedure has been developed with the use of a thermocycler. This procedure eliminates the need for preliminary experiments to determine the optimal temperature of hybridization for a given probe.

Original language	English (US)
Pages (from-to)	73-75
Number of pages	3
Journal	Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1385/mb:14:1:73
State	Published - 2000
Externally published	Yes

Keywords

RPA
Thermocycler

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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title = "Gradient temperature hybridization using a thermocycler for RNase protection assays",

abstract = "The RNase protection assay (RPA) is a sensitive and quantitative assay for the detection of specific RNAs. Because some probes require an optimal temperature for efficient hybridization to the target RNAs, preliminary experiments to determine the optimal temperatures have to be done empirically, a process that is laborious and time-consuming. In order to simplify this process, a new gradient temperature hybridization procedure has been developed with the use of a thermocycler. This procedure eliminates the need for preliminary experiments to determine the optimal temperature of hybridization for a given probe.",

keywords = "RPA, Thermocycler",

author = "Luwen Zhang",

note = "Funding Information: I thank Dr. Joseph S. Pagano for his support and encouragement; and Greg Buczynski for critical reading. This work was supported in part by grants from the National Institute of Allergy and Infectious Diseases (AI 42372-01) and from the National Cancer Institute (CA 19014) to JSP. LZ was supported by a NIH Individual National Research Service Award (5F 32 CA67433).",

year = "2000",

doi = "10.1385/mb:14:1:73",

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AU - Zhang, Luwen

N1 - Funding Information: I thank Dr. Joseph S. Pagano for his support and encouragement; and Greg Buczynski for critical reading. This work was supported in part by grants from the National Institute of Allergy and Infectious Diseases (AI 42372-01) and from the National Cancer Institute (CA 19014) to JSP. LZ was supported by a NIH Individual National Research Service Award (5F 32 CA67433).

PY - 2000

Y1 - 2000

N2 - The RNase protection assay (RPA) is a sensitive and quantitative assay for the detection of specific RNAs. Because some probes require an optimal temperature for efficient hybridization to the target RNAs, preliminary experiments to determine the optimal temperatures have to be done empirically, a process that is laborious and time-consuming. In order to simplify this process, a new gradient temperature hybridization procedure has been developed with the use of a thermocycler. This procedure eliminates the need for preliminary experiments to determine the optimal temperature of hybridization for a given probe.

AB - The RNase protection assay (RPA) is a sensitive and quantitative assay for the detection of specific RNAs. Because some probes require an optimal temperature for efficient hybridization to the target RNAs, preliminary experiments to determine the optimal temperatures have to be done empirically, a process that is laborious and time-consuming. In order to simplify this process, a new gradient temperature hybridization procedure has been developed with the use of a thermocycler. This procedure eliminates the need for preliminary experiments to determine the optimal temperature of hybridization for a given probe.

KW - RPA

KW - Thermocycler

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JO - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

JF - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

IS - 1

ER -

Gradient temperature hybridization using a thermocycler for RNase protection assays

Abstract

Keywords

ASJC Scopus subject areas

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