Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

The "omics" era began with transcriptomics and this progressed into proteomics. While useful, these approaches provide only circumstantial information about carbon flow, metabolic status, redox poise, etc. To more directly address these metabolic concerns, researchers have turned to the emerging field of metabolomics. In our laboratories, we frequently use NMR metabolomics to acquire a snapshot of bacterial metabolomes during stressful or transition events. Irrespective of the "omics" method of choice, the experimental outcome depends on the proper cultivation and preparation of bacterial samples. In addition, the integration of these large datasets requires that these cultivation conditions be clearly defined [1].

Original languageEnglish (US)
Title of host publicationStaphylococcus Epidermidis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages71-91
Number of pages21
ISBN (Print)9781627037358
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1106
ISSN (Print)1064-3745

Keywords

  • Bacterial growth
  • Growth conditions
  • Metabolism
  • Metabolomics
  • NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Somerville, G. A., & Powers, R. (2014). Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis. In Staphylococcus Epidermidis: Methods and Protocols (pp. 71-91). (Methods in Molecular Biology; Vol. 1106). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-736-5_6