Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


The "omics" era began with transcriptomics and this progressed into proteomics. While useful, these approaches provide only circumstantial information about carbon flow, metabolic status, redox poise, etc. To more directly address these metabolic concerns, researchers have turned to the emerging field of metabolomics. In our laboratories, we frequently use NMR metabolomics to acquire a snapshot of bacterial metabolomes during stressful or transition events. Irrespective of the "omics" method of choice, the experimental outcome depends on the proper cultivation and preparation of bacterial samples. In addition, the integration of these large datasets requires that these cultivation conditions be clearly defined [1].

Original languageEnglish (US)
Title of host publicationStaphylococcus Epidermidis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages21
ISBN (Print)9781627037358
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bacterial growth
  • Growth conditions
  • Metabolism
  • Metabolomics
  • NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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