Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae

James L. Van Etten; Dwight E. Burbank; Yuannan Xia; Russel H. Meints

doi:10.1016/0042-6822(83)90466-X

Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae

James L. Van Etten, Dwight E. Burbank, Yuannan Xia, Russel H. Meints

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Abstract

The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10^-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO₂ fixation of the host Chlorella.

Original language	English (US)
Pages (from-to)	117-125
Number of pages	9
Journal	Virology
Volume	126
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(83)90466-X
State	Published - Apr 15 1983

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Virology

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title = "Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae",

abstract = "The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.",

author = "{Van Etten}, {James L.} and Burbank, {Dwight E.} and Yuannan Xia and Meints, {Russel H.}",

note = "Funding Information: We thank Myron Brakke, Les Lane, R. L. Pardy, Dale Weis, Bob Schmidt, and Dave Coder for helpful discussions.T his investigationw as supportedi n part by the University of Nebraska-Lincoln Research Council and NIH BiomedicalR esearchS upport Grant RR-07055.",

year = "1983",

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doi = "10.1016/0042-6822(83)90466-X",

language = "English (US)",

volume = "126",

pages = "117--125",

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AU - Van Etten, James L.

AU - Burbank, Dwight E.

AU - Xia, Yuannan

AU - Meints, Russel H.

N1 - Funding Information: We thank Myron Brakke, Les Lane, R. L. Pardy, Dale Weis, Bob Schmidt, and Dave Coder for helpful discussions.T his investigationw as supportedi n part by the University of Nebraska-Lincoln Research Council and NIH BiomedicalR esearchS upport Grant RR-07055.

PY - 1983/4/15

Y1 - 1983/4/15

N2 - The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.

AB - The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.

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Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae

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