TY - JOUR
T1 - Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae
AU - Van Etten, James L.
AU - Burbank, Dwight E.
AU - Xia, Yuannan
AU - Meints, Russel H.
PY - 1983/4/15
Y1 - 1983/4/15
N2 - The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.
AB - The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.
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U2 - 10.1016/0042-6822(83)90466-X
DO - 10.1016/0042-6822(83)90466-X
M3 - Article
C2 - 18638936
AN - SCOPUS:0020615927
VL - 126
SP - 117
EP - 125
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -