Growth inhibition and differentiation of the human colon carcinoma cell line, Caco-2, by constitutive expression of insulin-like growth factor binding protein-3

Richard G. Macdonald, Beverly S. Schaffer, Il Jun Kang, Soon M. Hong, Eun J. Kim, Jung H.Y. Park

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The human colon carcinoma cell line, Caco-2, produces insulin-like growth factor binding protein-3 (IGFBP-3), the secretion of which correlates with markers of enterocyte differentiation. To investigate whether IGFBP-3 inhibits proliferation or induces differentiation, Caco-2 cells were stably transfected with an IGFBP-3 cDNA expression construct or pcDNA3 vector as a control. Accumulation of IGFBP-3 mRNA and secretion of the protein into conditioned medium 9 days after plating were readily detected in the transfected cells, whereas these parameters were undetectable in pcDNA3- transfected cells. Insulin-like growth factor binding protein-3-expressing cells grew at a rate similar to the controls for 6 days after plating, but achieved a much lower final density between days 10 and 12. By day 9 of culture, accumulation of sucrase-isomaltase mRNA, a marker of enterocytic differentiation of Caco-2 cells, was evident in the IGFBP-3-expressing cells, but was undetectable in the controls. These results indicate that IGFBP-3 may inhibit proliferation and induce early differentiation of Caco-2 cells.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume14
Issue number1
DOIs
StatePublished - Jan 1999

Keywords

  • Caco-2
  • Colon carcinoma
  • Differentiation
  • Insulin-like growth factor binding protein-3
  • Insulin-like growth factors
  • Markers of intestinal differentiation
  • Proliferation

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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