TY - JOUR
T1 - Growth of embryonal carcinoma cells in serum-free medium
AU - Rizzino, A.
AU - Sato, G.
PY - 1978
Y1 - 1978
N2 - Two mouse embryonal carcinoma cell lines, PCC.4 aza-1 and F9, have been grown in serum-free F-12 medium supplemented with Pedersen fetuin, insulin, transferrin, and 2-mercaptoethanol. This medium supports long-term growth of both cell lines. When these cells are transferred from medium containing serum to this serum-free medium, growth continues without any detectable lag. PCC.4 aza-1 grown in this medium for over 20 generations retains the capacity to differentiate in vivo. This medium appears to be a general serum-free medium for the growth of embryonal carcinoma cells.
AB - Two mouse embryonal carcinoma cell lines, PCC.4 aza-1 and F9, have been grown in serum-free F-12 medium supplemented with Pedersen fetuin, insulin, transferrin, and 2-mercaptoethanol. This medium supports long-term growth of both cell lines. When these cells are transferred from medium containing serum to this serum-free medium, growth continues without any detectable lag. PCC.4 aza-1 grown in this medium for over 20 generations retains the capacity to differentiate in vivo. This medium appears to be a general serum-free medium for the growth of embryonal carcinoma cells.
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U2 - 10.1073/pnas.75.4.1844
DO - 10.1073/pnas.75.4.1844
M3 - Article
C2 - 77018
AN - SCOPUS:0018141859
SN - 0027-8424
VL - 75
SP - 1844
EP - 1848
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4
ER -