Growth stimulation by transfection of intestinal epithelial cells with an antisense insulin-like growth factor-binding protein-2 construct

Mark R. Corkins, Jon A. Vanderhoof, Dorothy H. Slentz, Richard G. MacDonald, Jung H.Y. Park

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume211
Issue number3
DOIs
StatePublished - Jun 26 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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