Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells

Naseema M. Hoosein, Lars Thim, Klavs H. Jørgensen, Michael G. Brattain

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.

Original languageEnglish (US)
Pages (from-to)303-306
Number of pages4
JournalFEBS Letters
Volume247
Issue number2
DOIs
StatePublished - Apr 24 1989

Keywords

  • Protein, pS2
  • Vasoactive intestinal polypeptide
  • cyclic AMP level

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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