Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells

Naseema M. Hoosein; Lars Thim; Klavs H. Jørgensen; Michael G. Brattain

doi:10.1016/0014-5793(89)81357-2

Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells

Naseema M. Hoosein, Lars Thim, Klavs H. Jørgensen, Michael G. Brattain

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [³H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [³H]thymidine incorporation of 50-60% occurred at 3-300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [³H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.

Original language	English (US)
Pages (from-to)	303-306
Number of pages	4
Journal	FEBS Letters
Volume	247
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)81357-2
State	Published - Apr 24 1989
Externally published	Yes

Keywords

Protein, pS2
Vasoactive intestinal polypeptide
cyclic AMP level

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)81357-2

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title = "Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells",

abstract = "The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.",

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AU - Hoosein, Naseema M.

AU - Thim, Lars

AU - Jørgensen, Klavs H.

AU - Brattain, Michael G.

PY - 1989/4/24

Y1 - 1989/4/24

N2 - The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.

AB - The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.

KW - Protein, pS2

KW - Vasoactive intestinal polypeptide

KW - cyclic AMP level

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Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells

Abstract

Keywords

ASJC Scopus subject areas

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