TY - JOUR
T1 - Guidelines for animal exercise and training protocols for cardiovascular studies
AU - Poole, David C.
AU - Copp, Steven W.
AU - Colburn, Trenton D.
AU - Craig, Jesse C.
AU - Allen, David L.
AU - Sturek, Michael
AU - O’Leary, Donal S.
AU - Zucker, Irving H.
AU - Musch, Timothy I.
N1 - Funding Information:
T.D.C. was funded by National Heart, Lung, and Blood Institute (NHLBI) Grant F31-HL-145981. J.C.C. was funded by NHLBI Grant 2T32-HL-007576. S.W.C. was funded by NHLBI Grant HL-142877. T.I.M. was funded by NHLBI Grants HL-108328 and HL-142877 and American Heart Association (AHA) Grant-in-Aid Midwest Affiliate 4350011. D.S.O. was funded by NHLBI Grants HL-055473 and HL-126706. D.C.P was funded by NHLBI Grants HL-50306, HL-108328, and HL-137156 and AHA Grant-in-Aid Midwest Affiliate 4350011. M.S. was funded by NIH Grant DK-097512 and the Joshua Diabetes Research Fund. I.H.Z. was funded by NHLBI Grant P01-HL-62222 and the Theodore F. Hubbard Foundation.
Publisher Copyright:
© 2020 American Physiological Society. All rights reserved.
PY - 2020/5
Y1 - 2020/5
N2 - Poole DC, Copp SW, Colburn TD, Craig JC, Allen DL, Sturek M, O’Leary DS, Zucker IH, Musch TI. Guidelines for animal exercise and training protocols for cardiovascular studies. Am J Physiol Heart Circ Physiol 318: H1100 –H1138, 2020. First published March 20, 2020; doi:10.1152/ajpheart.00697.2019.—Whole body exercise tolerance is the consummate example of integrative physiological function among the metabolic, neuromuscular, cardiovascular, and respiratory systems. Depending on the animal selected, the energetic demands and flux through the oxygen transport system can increase two orders of magnitude from rest to maximal exercise. Thus, animal models in health and disease present the scientist with flexible, powerful, and, in some instances, purpose-built tools to explore the mechanistic bases for physiological function and help unveil the causes for pathological or age-related exercise intolerance. Elegant experimental designs and analyses of kinetic parameters and steady-state responses permit acute and chronic exercise paradigms to identify therapeutic targets for drug development in disease and also present the opportunity to test the efficacy of pharmacological and behavioral countermeasures during aging, for example. However, for this promise to be fully realized, the correct or optimal animal model must be selected in conjunction with reproducible tests of physiological function (e.g., exercise capacity and maximal oxygen uptake) that can be compared equitably across laboratories, clinics, and other proving grounds. Rigorously controlled animal exercise and training studies constitute the foundation of translational research. This review presents the most commonly selected animal models with guidelines for their use and obtaining reproducible results and, crucially, translates state-of-the-art techniques and procedures developed on humans to those animal models. Listen to this article’s corresponding podcast at: https://ajpheart.podbean.com/e/guidelines-for-animal-exercise-and-training-protocols/.
AB - Poole DC, Copp SW, Colburn TD, Craig JC, Allen DL, Sturek M, O’Leary DS, Zucker IH, Musch TI. Guidelines for animal exercise and training protocols for cardiovascular studies. Am J Physiol Heart Circ Physiol 318: H1100 –H1138, 2020. First published March 20, 2020; doi:10.1152/ajpheart.00697.2019.—Whole body exercise tolerance is the consummate example of integrative physiological function among the metabolic, neuromuscular, cardiovascular, and respiratory systems. Depending on the animal selected, the energetic demands and flux through the oxygen transport system can increase two orders of magnitude from rest to maximal exercise. Thus, animal models in health and disease present the scientist with flexible, powerful, and, in some instances, purpose-built tools to explore the mechanistic bases for physiological function and help unveil the causes for pathological or age-related exercise intolerance. Elegant experimental designs and analyses of kinetic parameters and steady-state responses permit acute and chronic exercise paradigms to identify therapeutic targets for drug development in disease and also present the opportunity to test the efficacy of pharmacological and behavioral countermeasures during aging, for example. However, for this promise to be fully realized, the correct or optimal animal model must be selected in conjunction with reproducible tests of physiological function (e.g., exercise capacity and maximal oxygen uptake) that can be compared equitably across laboratories, clinics, and other proving grounds. Rigorously controlled animal exercise and training studies constitute the foundation of translational research. This review presents the most commonly selected animal models with guidelines for their use and obtaining reproducible results and, crucially, translates state-of-the-art techniques and procedures developed on humans to those animal models. Listen to this article’s corresponding podcast at: https://ajpheart.podbean.com/e/guidelines-for-animal-exercise-and-training-protocols/.
KW - Critical speed
KW - Exercise tolerance
KW - Exhaustion
KW - Maximal oxygen uptake
KW - Oxygen transport system
UR - http://www.scopus.com/inward/record.url?scp=85084167688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084167688&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.00697.2019
DO - 10.1152/ajpheart.00697.2019
M3 - Review article
C2 - 32196357
AN - SCOPUS:85084167688
SN - 0363-6127
VL - 318
SP - H1100-H1138
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 5
ER -