Hairpin Loop Structure at the Termini of the Chlorella Virus PBCV-1 Genome

Yanping Zhang; Peter Strasser; Reingard Grabherr; James L. Van Etten

doi:10.1006/viro.1994.1444

Hairpin Loop Structure at the Termini of the Chlorella Virus PBCV-1 Genome

Yanping Zhang, Peter Strasser, Reingard Grabherr, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The termini of the chlorella virus PBCV-1 330-kb dsDNA genome consist of 35-nucleotide-long, incompletely base-paired, covalently closed hairpin loops that exist in one of two forms. The two forms are complementary when the 35 nucleotide sequences are inverted with respect to one another (flip and flop). Each hairpin loop structure is followed by an identical 2221-bp inverted repeat sequence after which the DNA sequence diverges. The strategy for cloning the PBCV-1 DNA hairpin ends may be useful for cloning other hairpin termini.

Original language	English (US)
Article number	71444
Pages (from-to)	1079-1082
Number of pages	4
Journal	Virology
Volume	202
Issue number	2
DOIs	https://doi.org/10.1006/viro.1994.1444
State	Published - Aug 1 1994

ASJC Scopus subject areas

Virology

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10.1006/viro.1994.1444

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abstract = "The termini of the chlorella virus PBCV-1 330-kb dsDNA genome consist of 35-nucleotide-long, incompletely base-paired, covalently closed hairpin loops that exist in one of two forms. The two forms are complementary when the 35 nucleotide sequences are inverted with respect to one another (flip and flop). Each hairpin loop structure is followed by an identical 2221-bp inverted repeat sequence after which the DNA sequence diverges. The strategy for cloning the PBCV-1 DNA hairpin ends may be useful for cloning other hairpin termini.",

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AB - The termini of the chlorella virus PBCV-1 330-kb dsDNA genome consist of 35-nucleotide-long, incompletely base-paired, covalently closed hairpin loops that exist in one of two forms. The two forms are complementary when the 35 nucleotide sequences are inverted with respect to one another (flip and flop). Each hairpin loop structure is followed by an identical 2221-bp inverted repeat sequence after which the DNA sequence diverges. The strategy for cloning the PBCV-1 DNA hairpin ends may be useful for cloning other hairpin termini.

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Hairpin Loop Structure at the Termini of the Chlorella Virus PBCV-1 Genome

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