TY - JOUR
T1 - Heat shock protein 27 is expressed in normal and malignant human melanocytes in vivo
AU - Kang, Steven H.
AU - Fung, Maxwell A.
AU - Gandour-Edwards, Regina
AU - Reilly, Debra
AU - Dizon, Teresa
AU - Grahn, Jennifer
AU - Isseroff, R. Rivkah
PY - 2004/11
Y1 - 2004/11
N2 - Background: Heat shock proteins (HSPs) are a family of highly conserved proteins found ubiquitously in mammalian cells, believed to be regulators of normal cell physiology and the cellular stress response. In addition, the small 27-kDa heat shock protein (HSP27) has previously been found to be a differentiation marker for keratinocytes and a prognostic marker associated with increased survival in certain cancerous tumors. Methods: Using immunohistochemistry on routinely processed paraffin sections, we examined skin biopsies from 15 invasive melanomas, 13 intradermal nevi, and two compound nevi immunostained with a mouse monoclonal antibody to HSP27. In addition, cultured melanocytes were heat stressed at 45°C for 1 h and then fixed and immunostained in order to localize HSP27 expression intracellularly. Results: We found cytoplasmic and strong perinuclear staining of HSP27 in melanocytes in normal skin, in melanomas, and in nevi. Nuclear reactivity was absent. In addition, in cultured non-malignant melanocytes, HSP27 expression relocated from the cytoplasm to the nucleus with heat stress. Conclusions: To our knowledge, this investigation is the first to demonstrate that HSP27 is expressed in melanocytes in normal skin, in nevi, and in non-malignant cultured melanocytes.
AB - Background: Heat shock proteins (HSPs) are a family of highly conserved proteins found ubiquitously in mammalian cells, believed to be regulators of normal cell physiology and the cellular stress response. In addition, the small 27-kDa heat shock protein (HSP27) has previously been found to be a differentiation marker for keratinocytes and a prognostic marker associated with increased survival in certain cancerous tumors. Methods: Using immunohistochemistry on routinely processed paraffin sections, we examined skin biopsies from 15 invasive melanomas, 13 intradermal nevi, and two compound nevi immunostained with a mouse monoclonal antibody to HSP27. In addition, cultured melanocytes were heat stressed at 45°C for 1 h and then fixed and immunostained in order to localize HSP27 expression intracellularly. Results: We found cytoplasmic and strong perinuclear staining of HSP27 in melanocytes in normal skin, in melanomas, and in nevi. Nuclear reactivity was absent. In addition, in cultured non-malignant melanocytes, HSP27 expression relocated from the cytoplasm to the nucleus with heat stress. Conclusions: To our knowledge, this investigation is the first to demonstrate that HSP27 is expressed in melanocytes in normal skin, in nevi, and in non-malignant cultured melanocytes.
UR - http://www.scopus.com/inward/record.url?scp=7444220648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7444220648&partnerID=8YFLogxK
U2 - 10.1111/j.0303-6987.2004.00248.x
DO - 10.1111/j.0303-6987.2004.00248.x
M3 - Article
C2 - 15491326
AN - SCOPUS:7444220648
SN - 0303-6987
VL - 31
SP - 665
EP - 671
JO - Journal of Cutaneous Pathology
JF - Journal of Cutaneous Pathology
IS - 10
ER -