Hepatic mitochondrial efficiency in lines of mice differing in feed intake

T. W. Murphy, J. M. Mcdonald, M. K. Nielsen

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Selection was practiced for high (MH) and low (ML) heat loss using direct calorimetry to create lines of mice differing in feed intake per unit average BW (FI/BW). A total of 25 generations of selection was practiced during maintenance of an unselected control (MC). As a percentage of MC, mice of MH and ML lines differed in heat loss and FI/BW by 56 and 34%, respectively, when selection ceased. The purpose of this study was to determine hepatic mitochondrial efficiency in mice at maintenance across the genetic lines. After measurement of feed intakeand BW over a 2-wk period, mice were euthanized, and their livers were extracted. Livers were homogenized and mitochondria were iso lated in buffer for measurement of oxygen consumption and, hence, mitochondrial activity using a Clark-type oxygen electrode. Mitochondria of ML mice expressed greater (P < 0.03) respiratory control ratio by 22% than that for MH mice, revealing greater degree of uncoupling during electron transport in MH mice. Difference in FI/BW (P < 0.001) was large with MH mice consuming 34% more than ML mice. The respiratory control ratio differed between mice of MH and ML lines as expected; however, regression analysis revealed that it explained essentially no variation in feed intake per BW.

Original languageEnglish (US)
Pages (from-to)2077-2082
Number of pages6
JournalJournal of animal science
Issue number5
StatePublished - May 2013


  • Feed intake
  • Liver
  • Mice
  • Mitochondria
  • Respiratory control ratio
  • Uncoupling

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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