Herpes simplex virus as a transneuronal tracer

Robert B. Norgren, Michael N. Lehman

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Determining the connections of neural systems is critical for determining how they function. In this review, we focus on the use of HSV-1 and HSV-2 as transneuronal tracers. Using HSV to examine neural circuits is technically simple. HSV is injected into the area of interest, and after several days, the animals are perfused and processed for immunohistochemistry with antibodies to HSV proteins. Variables which influence HSV infection include species of host, age of host, titre of virus, strain of virus and phenotype of infected cell. The choice of strain of HSV is critically important. Several strains of HSV-1 and HSV-2 have been utilized for purposes of transneuronal tract-tracing. HSV has been used successfully to study neuronal circuitry in a variety of different neuroanatomical systems including the somatosensory, olfactory, visual, motor, autonomic and limbic systems.

Original languageEnglish (US)
Pages (from-to)695-708
Number of pages14
JournalNeuroscience and Biobehavioral Reviews
Issue number6
StatePublished - Oct 1998


  • Amygdala
  • Autonomic
  • HSV
  • Herpes simplex virus
  • Hypothalamus
  • Limbi c
  • Olfactory
  • Retina
  • Somatosensory
  • Tracer
  • Transneuronal
  • Visual

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience


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