Heterogeneity of Cytokine and Growth Factor Gene Expression in Human Melanoma Cells with Different Metastatic Potentials

Rakesh K. Singh, Mordechai Gutman, Robert Radinsky

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The purpose of this study was to determine the mRNA expression level of multiple cytokine and growth factor genes in human malignant melanoma. Melanoma cells were isolated from several surgical specimens, adapted to growth in culture, characterized for their ability to produce experimental metastases in nude mice, and assessed for cytokine and growth factor steady-state gene expression. Highly metastatic in vivo- and in vitro-derived variants isolated from a single melanoma, A375, were also analyzed. Northern blot analyses revealed that all melanomas analyzed constitutively expressed steady-state mRNA transcripts for the growth and angiogenic factors, basic fibroblast growth factor (bFGF), and transforming growth factor α (TGF-α), which correlated with metastatic propensity. Only one highly metastatic melanoma, TXM-1, originally isolated from a lymph node metastasis, expressed mRNA transcripts specific for monocyte chemotactic and activating factor (MCAF) and granulocyte-macrophage colony-stimulating factor (GM-CSF). Similarly, of the nine melanomas examined, only TXM-1 expressed interleukin (IL)-1α, IL-1β, and IL-6, important immuno-modulatory cytokines. These data demonstrate the differential and heterogeneous expression of cytokine and growth factor genes in human malignant melanoma.

Original languageEnglish (US)
Pages (from-to)81-87
Number of pages7
JournalJournal of Interferon and Cytokine Research
Volume15
Issue number1
DOIs
StatePublished - Jan 1995

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

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