Heterologous phasin expression in Rhodopseudomonas palustris CGA009 for bioplastic production from lignocellulosic biomass

Brandi Brown, Cheryl Immethun, Adil Alsiyabi, Dianna Long, Mark Wilkins, Rajib Saha

Research output: Contribution to journalArticlepeer-review

Abstract

Rhodopseudomonas palustris CGA009 is a metabolically robust microbe that can utilize lignin breakdown products to produce polyhydroxyalkanoates (PHAs), biopolymers with the potential to replace conventional plastics. Our recent efforts suggest PHA granule formation is a limiting factor for maximum production of the bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by R. palustris. The Phap1 phasin (phaP1) from the PHB-producing model bacterium Cupriavidus necator H16 was expressed in R. palustris with the aim of overproducing PHBV from the lignin breakdown product p-coumarate by fostering smaller and more abundant granules. Expression of phaP1 yielded PHBV production from R. palustris aerobically (0.7 g/L), which does not occur in the wild-type strain, and led to a significantly higher PHBV titer than wild-type anaerobic production (0.41 g/L). The 3HV fractions were also significantly increased under both anaerobic and aerobic conditions, which boosts thermomechanical properties and potential for application. Thus, heterologous phasin expression in R. palustris provides flexibility for industrial processing and could foster compositional changes in copolymers with better thermomechanical properties compared to PHB alone.

Original languageEnglish (US)
Article numbere00191
JournalMetabolic Engineering Communications
Volume14
DOIs
StatePublished - Jun 2022

Keywords

  • Bioplastic
  • Lignin
  • Lignocellulosic
  • Phasin
  • Polyhydroxybutyrate
  • Rhodopseudomonas palustris

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Heterologous phasin expression in Rhodopseudomonas palustris CGA009 for bioplastic production from lignocellulosic biomass'. Together they form a unique fingerprint.

Cite this