HIF-dependent regulation of AKAP12 (gravin) in the control of human vascular endothelial function

Thomas Weissmüller, Louise E. Glover, Blair Fennimore, Valerie F. Curtis, Christopher F. MacManus, Stefan F. Ehrentraut, Eric L. Campbell, Melanie Scully, Bryon D. Grove, Sean P. Colgan

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Hypoxia has been widely implicated in many pathological conditions, including those associated with inflammation and tumorigenesis. A number of recent studies have implicated hypoxia in the control of vasculogenesis and permeability, the basis for which is not fully understood. Here we examine the transcriptional regulation of angiogenesis and permeability by hypoxia in endothelial cells. Guided by a global profiling approach in cultured endothelial cells, these studies revealed the selective induction of human gravin (protein kinase A anchoring protein 12) by hypoxia. Analysis of the cloned gravin promoter identified a functional hypoxia-responsive region including 2 binding sites for hypoxia-inducible factor (HIF). Site-directed mutagenesis identified the most distal HIF-binding site as essential for the induction of gravin by hypoxia. Further studies examining gravin gain and loss of function confirmed strong dependence of gravin in control of microvascular endothelial tube formation, wherein gravin functions as a "braking" system for angiogenesis. Additional studies in confluent endothelia revealed that gravin functionally couples to control endothelial barrier function in response to protein kinase A (PKA) agonists. Taken together, these results demonstrate transcriptional coordination of gravin by HIF-1α and amplified PKA-dependent endothelial responses. These findings provide an important link between hypoxia and metabolic conditions associated with inflammation and angiogenesis.

Original languageEnglish (US)
Pages (from-to)256-264
Number of pages9
JournalFASEB Journal
Volume28
Issue number1
DOIs
StatePublished - Jan 2014
Externally publishedYes

Keywords

  • Angiogenesis
  • Barrier function
  • Hypoxia-inducible factor
  • Permeability

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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