High-frequency transfection of CHO cells using polybrene

William G. Chaney; Daniel R. Howard; Jeffrey W. Pollard; Sandra Sallustio; Pamela Stanley

doi:10.1007/BF01570782

High-frequency transfection of CHO cells using polybrene

William G. Chaney, Daniel R. Howard, Jeffrey W. Pollard, Sandra Sallustio, Pamela Stanley

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126 Scopus citations

Abstract

High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

Original language	English (US)
Pages (from-to)	237-244
Number of pages	8
Journal	Somatic Cell and Molecular Genetics
Volume	12
Issue number	3
DOIs	https://doi.org/10.1007/BF01570782
State	Published - May 1986
Externally published	Yes

ASJC Scopus subject areas

Genetics
Cell Biology

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10.1007/BF01570782

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title = "High-frequency transfection of CHO cells using polybrene",

abstract = "High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.",

author = "Chaney, {William G.} and Howard, {Daniel R.} and Pollard, {Jeffrey W.} and Sandra Sallustio and Pamela Stanley",

year = "1986",

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language = "English (US)",

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AU - Chaney, William G.

AU - Howard, Daniel R.

AU - Pollard, Jeffrey W.

AU - Sallustio, Sandra

AU - Stanley, Pamela

PY - 1986/5

Y1 - 1986/5

N2 - High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

AB - High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

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High-frequency transfection of CHO cells using polybrene

Abstract

ASJC Scopus subject areas

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